Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysis.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 64 ·No. 2 ·1983-01-00 ·Pages 117-8

Uchimiya H, Ohgawara T, Kato H, Akiyama T, Harada H, Sugiura M

Abstract

Restriction endonucleases discriminated between the rDNAs contained in callus tissues derived from Nicotiana glauca, N. langsdorffii, and their somatic hybrids produced by protoplast fusion. With XbaI, a single repeat fragment of 7.5 × 10(6) daltons was produced from N. glauca rDNA compared to a single repeat fragment of 4.2 × 10(6) daltons produced from N. langsdorffii rDNA. Both kinds of XbaI fragments were found in somatic hybrids.

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Uchimiya H

Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Japan.

Ohgawara T

Kato H

Akiyama T

Harada H

Sugiura M

References (4)

4 references, click to expand

Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Parasexual interspecific plant hybridization.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2292-4 PMID: 16592009
Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells.
Plant Physiol. 1974 Dec;54(6):936-44 PMID: 16659004
Somatic hybridization between male sterile Nicotiana tabacum and N. glutinosa through protoplast fusion.
Theor Appl Genet. 1982 Mar;61(1):69-72 PMID: 24271377

Article Info

Journal

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Abbr.

Theor Appl Genet

ISSN

0040-5752

DOI

10.1007/BF00272717

Published

1983-01-00

Pages

117-8

Language

English

Region

Germany

NLM ID

0145600

External Links

PubMed source DOI: 10.1007/BF00272717 View journal details

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Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysis.

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