Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 59 ·No. 3 ·1981-03-00 ·Pages 191-5

Menczel L, Nagy F, Kiss ZR, Maliga P

Abstract

Protoplasts of Nicotiana tabacum SRI (streptomycin resistant) and of Nicotiana knightiana (streptomycin sensitive) were fused using polyethylene glycol treatment. From three heterokaryons 500 clones were obtained. From the 43 which were further investigated, 6 resistant, 3 sensitive, and 34 chimeric (consisting of resistant and sensitive sectors) calli were found. From eight clones, a total of 39 plants were regenerated and identified as somatic hybrids. Chloroplast type (N. tabacum = NT or N. knightiana = NK) in the plants was determined on the basis of the species specific EcoRI restriction pattern of the chloroplast DNA. Regenerates contained NT (13 plants) or NK (15 plants) plastids but only the plants with the NT chloroplasts were resistant to streptomycin. This finding and our earlier data on uniparental inheritance points to the chloroplasts as the carriers of the streptomycin resistance factor.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Menczel L

Institute of Plant Physiology, BRC, Hungarian Academy of Sciences, Szeged, Hungary.

Nagy F

Kiss Z R

Maliga P

References (10)

10 references, click to expand

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Article Info

Journal

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Abbr.

Theor Appl Genet

ISSN

0040-5752

DOI

10.1007/BF00264975

Published

1981-03-00

Pages

191-5

Language

English

Region

Germany

NLM ID

0145600

External Links

PubMed source DOI: 10.1007/BF00264975 View journal details

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Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids.

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