Isolation of a cDNA clone for the acyl carrier protein-I of spinach.

Plant molecular biology ·Vol. 9 ·No. 2 ·1987-03-00 ·Pages 127-34

Scherer DE, Knauf VC

Abstract

A 715 base pair cDNA clone coding for an acyl carrier protein (ACP) in spinach leaves has been isolated and characterized. The amino acid sequence indicated by the cDNA sequence closely matches the amino acid sequence of the ACP-I isoform. The presence of polyadenylation and DNA sequence coding for a precursor protein with a putative transit peptide, and the absence of hybridization between the cloned DNA and isolated spinach plastid DNA collectively show that the ACP-I gene is nuclear-encoded. The ACP-I cloned DNA did not cross-hybridize with mRNA from spinach tissues in which ACP-II has been found. Cross-hybridization with mRNA from tissues of Brassica campestris was either weak or undetectable. The cloning of an ACP-I gene represents an initial step in the molecular dissection of fatty acid synthetase in plants.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Scherer D E

Calgene, Inc., 1920 Fifth Street, 95616, Davis, CA, USA.

Knauf V C

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00015645

Published

1987-03-00

Pages

127-34

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF00015645 View journal details

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Isolation of a cDNA clone for the acyl carrier protein-I of spinach.

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