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PMID: 2428838 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a 16,600-dalton outer membrane protein on nontypeable Haemophilus influenzae as a target for human serum bactericidal antibody.

The Journal of clinical investigation ·Vol. 78 ·No. 4 ·1986-10-00 ·Pages 1020-7

Murphy TF, Bartos LC, Rice PA, Nelson MB, Dudas KC, Apicella MA

Abstract

A 16,600-D outer membrane protein is present in all strains of Haemophilus influenzae and antibodies to this protein are present in human serum. This study was designed to assess the role of this outer membrane protein (P6) in nontypeable H. influenzae as a target for human serum bactericidal antibody. P6 was isolated and coupled to an affinity column. Depleting normal human serum of antibodies to P6 by affinity chromatography resulted in reduced bactericidal activity of that serum for nontypeable H. influenzae. Immunopurified antibodies to P6 from human serum were bactericidal. Finally, preincubation of bacteria with a monoclonal antibody that recognizes a surface epitope on P6, inhibited human serum bactericidal killing. Taken together, these experiments establish that P6 is a target for human bactericidal antibodies. This observation provides evidence that P6 plays a potentially important role in human immunity to infection by nontypeable H. influenzae.

MeSH Terms
Antibodies, Bacterial/analysis Antibodies, Monoclonal Blood Bactericidal Activity Chromatography, Affinity Enzyme-Linked Immunosorbent Assay Epitopes/analysis Haemophilus influenzae/analysis Membrane Proteins/analysis,isolation & purification Molecular Weight
Chemicals
Antibodies, Bacterial Antibodies, Monoclonal Epitopes Membrane Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Murphy T F
Bartos L C
Rice P A
Nelson M B
Dudas K C
Apicella M A
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31 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112656
Published
1986-10-00
Pages
1020-7
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423749
Subset
IM
Grants
NIAID NIH HHS · AI 19641 · United States
External Links
PubMed source DOI: 10.1172/JCI112656 PMC Full Text View journal details
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