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PMID: 2429318 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Association of La and Ro antigens with intracellular structures in HEp-2 carcinoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 20 ·1986-10-00 ·Pages 7770-4

Bachmann M, Mayet WJ, Schröder HC, Pfeifer K, Meyer zum Büschenfelde KH, Müller WE

Abstract

Monoclonal antibodies were raised against homogeneous Ro and La antigens, two proteins associated with Ro and La ribonucleoproteins (RNPs). The specificity of the monoclonal antibodies was proven by immunoblot analysis and by immunoprecipitation. The anti-Ro antibody reacted with a Mr 95,000 protein in a mouse lymphoma cell extract and with a Mr 60,000 polypeptide in extracts from human spleen. The anti-La antibody recognized a Mr 50,000 polypeptide in the mouse L5178y cell extract. The two monoclonal antibodies precipitated RNPs that contained the typical RNA species of Ro or La RNPs. The localization of Ro and La antigen was performed by direct immunofluorescence microscopy. It was found that the anti-Ro antibody reacted with a fibrous network that behaves like cytokeratin, one of the intermediate filament systems. The anti-La antibody reacted with nuclear structures that gave a speckled-type pattern.

MeSH Terms
Animals Antibodies, Antinuclear/immunology Antibodies, Monoclonal/immunology Autoantigens/analysis Cell Line Cell Nucleus/immunology Female Fluorescent Antibody Technique Humans Intermediate Filaments/immunology Keratins/immunology Mice Mice, Inbred BALB C Molecular Weight Neoplasms, Experimental/immunology RNA, Small Cytoplasmic Ribonucleoproteins
Chemicals
Antibodies, Antinuclear Antibodies, Monoclonal Autoantigens RNA, Small Cytoplasmic RO60 protein, human Ribonucleoproteins SS-A antibodies SS-A antigen SS-B antibodies SS-B antigen Keratins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bachmann M
Mayet W J
Schröder H C
Pfeifer K
Meyer zum Büschenfelde K H
Müller W E
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.20.7770
Published
1986-10-00
Pages
7770-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386803
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.20.7770 PMC Full Text View journal details
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