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PMID: 2430111 Published · ppublish English Journal Article

Structural characterization of reverse transcriptase and endonuclease polypeptides of the acquired immunodeficiency syndrome retrovirus.

Journal of virology ·Vol. 60 ·No. 2 ·1986-11-00 ·Pages 771-5

Lightfoote MM, Coligan JE, Folks TM, Fauci AS, Martin MA, Venkatesan S

Abstract

Automated N-terminal microsequencing of immune affinity-purified acquired immunodeficiency syndrome retrovirus polypeptides from infected cells was used to locate the N termini of 64-, 51-, and 34-kilodalton (kDa) polypeptides within the pol open reading frame (ORF) of the proviral DNA. The 64- and 51-kDa proteins had identical N termini (Pro-Ile-Ser-Pro-IIe-Glu-Thr-Val-) positioned 156 residues from the beginning of the pol ORF. The N terminus of the 34-kDa pol gene product, Phe-Leu-Asp-Gly-Ile-Asp-Lys-, mapped 716 residues into the pol ORF. These polypeptides were absent in an RT-negative, CD4-negative, persistently infected cell line (8E5) carrying a single defective copy of a constitutively expressed, integrated proviral DNA.

MeSH Terms
Amino Acid Sequence Cell Line Endonucleases/genetics Genes, Viral HIV/enzymology,genetics Molecular Weight RNA-Directed DNA Polymerase/genetics
Chemicals
RNA-Directed DNA Polymerase Endonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lightfoote M M
Coligan J E
Folks T M
Fauci A S
Martin M A
Venkatesan S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.2.771-775.1986
Published
1986-11-00
Pages
771-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288955
Subset
IM
Databases
GENBANK
K03455, M14124, M14125
External Links
PubMed source DOI: 10.1128/JVI.60.2.771-775.1986 PMC Full Text View journal details
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