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PMID: 24306377 Published · ppublish English Journal Article

Microtubules and coated vesicles in guard-cell protoplasts ofAllium cepa L.

Planta ·Vol. 149 ·No. 4 ·1980-01-00 ·Pages 389-401

Doohan ME, Palevitz BA

Abstract

Protoplasts were prepared from the guard cells ofA. cepa. Epidermal peels taken from expanding green leaves and largely free of mesophyll were treated with Cellulysin, and protoplasts were harvested after 18 h of digestion. That the protoplasts were derived from guard cells was ascertained from their characteristic vacuolar autofluorescence and from observations showing that all other epidermal cells are killed in the peeling procedure. The protoplasts proved to be a good system with which to view the cell cortex and inner surface of the plasmalemma. The lysis of cells adhering to polylysine-treated, Formvar-coated grids, followed by negative staining in uranyl acetate, showed that many microtubules normally present in ordered arrays in situ remain closely applied to the inner surface of the plasmalemma in protoplasts. In addition, numerous vesiculate elements including coated vesicles and/or pits are present amongst the microtubules. Similar vesicles are evident in thin sections of fixed, embedded guard cells and protoplasts. The significance of these structures in the cell cortex is discussed.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Doohan M E
Department of Botany, University of Georgia, 30602, Athens, GA, USA.
Palevitz B A
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/BF00571175
Published
1980-01-00
Pages
389-401
Language
English
Region
Germany
NLM ID
1250576
External Links
PubMed source DOI: 10.1007/BF00571175 View journal details
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