Genes and transcripts for the P700 chlorophylla apoprotein and subunit 2 of the photosystem I reaction center complex from spinach thylakoid membranes.

Plant molecular biology ·Vol. 2 ·No. 2 ·1983-03-00 ·Pages 95-107

Westhoff P, Alt J, Nelson N, Bottomley W, Bünemann H, Herrmann RG

Abstract

A photosystem I reaction center complex has been purified to homogeneity by a procedure involving partial solubilization of spinach thylakoid membranes, ion exchange chromatography and centrifugation in sucrose gradients. The complex contains 7 polypeptides: the P700 chlorophylla apoprotein with an apparent molecular weight of 67 kd, which at high resolution splits into two bands, and smaller polypeptides of 22 (subunit 2), 18.5, 18, 16, 12 and 10 kd.Stable transcripts for the P700 chlorophylla apojprotein and subunit 2 were found in plastid and cytosolic RNA, respectively. The apoprotein product obtained by translation in a mRNA-dependent cell-free rabbit reticulocyte lysate and also by DNA-programmed transcription-translation of cloned plastid DNA fragments inE. coli lysates was indistinguishable immunologically and electrophoretically from the authentic protein. However, the product immunologically related to subunit 2 was 4 kd larger than the mature compound indicating that this protein is encoded in the nucleus and synthesized as a precursor.The gene for the P700 chlorophylla apoprotein has been physically mapped on the spinach plastid chromosome by hybrid selection mapping and DNA-programmed cell-free transcription-translation using cloned restriction fragments of plastid DNA. There is one gene copy per chromosome and it is located centrally in the large single-copy region of the circular DNA molecule. This gene is uninterrupted and is transcribed in the same direction as that of the large subunit of ribulose bisphosphate carboxylase/oxygenase. Its transcript is approximately 4 kb longer than the 2 kbp structural gene.

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Westhoff P

Botanisches Institut der Universität, 4, Düsseldorf 1, F.R.G..

Alt J

Nelson N

Bottomley W

Bünemann H

Herrmann R G

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF01595171

Published

1983-03-00

Pages

95-107

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF01595171 View journal details

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Genes and transcripts for the P700 chlorophylla apoprotein and subunit 2 of the photosystem I reaction center complex from spinach thylakoid membranes.

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