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PMID: 2432001 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Restricted light chain subgroup expression on human rheumatoid factor paraproteins determined by monoclonal antibodies.

Immunology ·Vol. 59 ·No. 3 ·1986-11-00 ·Pages 473-8

Mageed RA, Walker MR, Jefferis R

Abstract

Two hybridoma antibodies specific for determinants on the V kappa light chain subgroup have been produced and characterized. Antibodies C7 and B12 reacted with distinct V kappa epitopes irrespective of association with heavy chain class or subclass. Epitopes recognized by C7 and B12 were expressed on the light chain of IgG, IgA, and IgM and Bence-Jones paraproteins from the V kappa subgroup. However, a preferential association of both epitopes with IgM RF paraproteins was demonstrated. Hybridomas C7 and B12 reacted with 12/12 and 10/12 IgM RF paraproteins, respectively, but only with 3/6 IgM paraproteins, with no RF activity. Both epitopes C7 and B12, were immunodominant and conformation dependent, being detected by HA, HAI and ELISA on intact light chain but not isolated VK.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Epitopes/immunology Humans Immunoglobulin Light Chains/immunology Immunoglobulin M/immunology Immunoglobulin Variable Region/immunology Mice Mice, Inbred BALB C Paraproteins/immunology Rheumatoid Factor/immunology
Chemicals
Antibodies, Monoclonal Epitopes Immunoglobulin Light Chains Immunoglobulin M Immunoglobulin Variable Region Paraproteins Rheumatoid Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mageed R A
Walker M R
Jefferis R
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20 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1986-11-00
Pages
473-8
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1453195
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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