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PMID: 2435757 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of the thrombospondin membrane receptor.

The Journal of clinical investigation ·Vol. 79 ·No. 4 ·1987-04-00 ·Pages 1054-61

Asch AS, Barnwell J, Silverstein RL, Nachman RL

Abstract

Thrombospondin (TSP), a 450-kD multifunctional glycoprotein with a broad tissue distribution, is secreted upon platelet stimulation, binds to the activated platelet surface, and supports platelet aggregation. We have identified and isolated an 88-kd membrane glycoprotein present in platelets, endothelial cells, monocytes, and a variety of human tumor cell lines that is the membrane binding site for TSP. Endogenous platelet TSP binding to thrombin- and ionophore-stimulated human platelets was inhibited in the presence of the monoclonal antibody OKM5. TSP binding to C32 melanoma cells and HT1080 fibrosarcoma cells was specific and also inhibitable with OKM5 Mab. Cell labeling followed by specific immunoprecipitation demonstrated biosynthesis of a single 88-kD glycoprotein. Binding of TSP to the isolated membrane protein was specific and saturable. These studies identify an 88-kD membrane glycoprotein that reacts with the monoclonal antibody, OKM5, and may function as the cellular TSP receptor.

MeSH Terms
Antibodies, Monoclonal Blood Platelets/chemistry,drug effects CD36 Antigens Cell Line Fibrosarcoma/chemistry Glycoproteins/metabolism Humans Melanoma/chemistry Membrane Proteins/isolation & purification Molecular Weight Receptors, Mitogen/isolation & purification Thrombin/pharmacology Thrombospondins
Chemicals
Antibodies, Monoclonal CD36 Antigens Glycoproteins Membrane Proteins Receptors, Mitogen Thrombospondins Thrombin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Asch A S
Barnwell J
Silverstein R L
Nachman R L
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112918
Published
1987-04-00
Pages
1054-61
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC424283
Subset
IM
Grants
NHLBI NIH HHS · HL-01567-01 · United States
NHLBI NIH HHS · HL-18828 · United States
External Links
PubMed source DOI: 10.1172/JCI112918 PMC Full Text View journal details
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