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PMID: 2437321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection.

Journal of virology ·Vol. 61 ·No. 5 ·1987-05-00 ·Pages 1690-4

Kwok S, Mack DH, Mullis KB, Poiesz B, Ehrlich G, Blair D, Friedman-Kien A, Sninsky JJ

Abstract

Human immunodeficiency virus (HIV) has been associated with acquired immunodeficiency syndrome and related disorders. Assays to detect antibodies to HIV proteins have been developed and used to screen sera for the identification of individuals who have been exposed to the virus. Although these serological tests have significant sensitivity and specificity for detecting exposure to the virus, they do not provide direct identification of HIV. We report here the application of recently developed nucleic acid amplification and oligonucleotide-based detection procedures for the identification of HIV sequences in established infected cell lines and in cells cultured from infected individuals.

MeSH Terms
Acquired Immunodeficiency Syndrome/diagnosis,microbiology Cell Line DNA Restriction Enzymes DNA, Viral/analysis HIV/analysis,genetics Nucleic Acid Hybridization Oligodeoxyribonucleotides RNA-Directed DNA Polymerase/analysis
Chemicals
DNA, Viral Oligodeoxyribonucleotides RNA-Directed DNA Polymerase DNA Restriction Enzymes
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kwok S
Mack D H
Mullis K B
Poiesz B
Ehrlich G
Blair D
Friedman-Kien A
Sninsky J J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.5.1690-1694.1987
Published
1987-05-00
Pages
1690-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254157
Subset
IM
Grants
NCI NIH HHS · CA37478 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.5.1690-1694.1987 PMC Full Text View journal details
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