Home LiteratureArticle Details
PMID: 2440025 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Production of a monoclonal antibody useful in the molecular characterization of murine T-cell-replacing factor/B-cell growth factor II.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 13 ·1987-07-00 ·Pages 4581-5

Harada N, Takahashi T, Matsumoto M, Kinashi T, Ohara J, Kikuchi Y, Koyama N, Severinson E, Yaoita Y, Honjo T

Abstract

T-cell-replacing factor (TRF) is a T-cell-derived factor required for terminal differentiation of activated B cells to immunoglobulin-secreting cells. Previous studies have shown that a murine T-cell hybrid (B151K12) produces factor(s) that (i) induce immunoglobulin secretion by the B-cell leukemia line BCL1 and secondary anti-2,4-dinitrophenyl IgG synthesis in vitro by dinitrophenyl-primed B cells (TRF activity) and (ii) cause proliferation of the BCL1 cells [B-cell growth factor II (BCGF-II) activity]. Both activities appear to be associated with the same molecule. Here we report the production of a monoclonal antibody to murine TRF. The monoclonal antibody, designated TB13, strongly inhibited both TRF and BCGF-II activities and absorbed TRF- as well as BCGF-II-active molecules produced by B151K12 and by Xenopus oocytes that had been injected with mRNA transcribed from plasmid pSP6K-mTRF23. Inhibition was linearly dependent on the concentration of both TB13 and TRF. Monoclonal antibody TB13 did not inhibit the activities of B-cell stimulatory factor 1, interleukin 1, interleukin 2, or interleukin 3. TRF activity in dissolved samples of immunoprecipitates obtained with TB13 was recovered after NaDodSO4/PAGE, in the fractions corresponding to a protein band at Mr 46,000. Our results indicate that monoclonal antibody TB13 recognizes a molecule that has both TRF and BCGF-II activities.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibody Specificity B-Lymphocytes/drug effects,immunology Cell Line Chromatography, Affinity Epitopes/immunology Female Growth Substances/immunology,pharmacology Interleukin-4 Interleukin-5 Lymphokines/immunology,pharmacology Mice Mice, Inbred BALB C Mice, Inbred C3H Molecular Weight Rats Rats, Inbred Strains Recombinant Proteins/pharmacology
Chemicals
Antibodies, Monoclonal Epitopes Growth Substances Interleukin-5 Lymphokines Recombinant Proteins Interleukin-4
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Harada N
Takahashi T
Matsumoto M
Kinashi T
Ohara J
Kikuchi Y
Koyama N
Severinson E
Yaoita Y
Honjo T
References (31)
31 references, click to expand
  1. B cell growth and differentiation factors.
    Immunol Rev. 1984 Apr;78:185-210 PMID: 6429034
  2. Purification and physicochemical characterization of murine T cell replacing factor (TRF).
    J Immunol. 1985 Jan;134(1):382-9 PMID: 3871109
  3. T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1612-6 PMID: 6966402
  4. Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1.
    Nature. 1985 May 23-29;315(6017):333-6 PMID: 2582266
  5. Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II.
    Nature. 1986 Nov 6-12;324(6092):70-3 PMID: 3024009
  6. Antigen-induced T cell-replacing factor (TRF). I. Functional characterization of a TRF-producing helper T cell subset and genetic studies on TRF production.
    J Immunol. 1980 May;124(5):2414-22 PMID: 6965971
  7. T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.
    J Exp Med. 1982 Mar 1;155(3):734-48 PMID: 7038025
  8. Mediators from cloned T helper cell lines affect immunoglobulin expression by B cells.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4756-60 PMID: 6812054
  9. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  10. Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter.
    Nature. 1986 Feb 20-26;319(6055):640-6 PMID: 3005865
  11. Regulation of B-cell differentiation: interactions of factors and corresponding receptors.
    Annu Rev Immunol. 1986;4:167-204 PMID: 2939856
  12. Induction of B cell differentiation by T cell factors. I. Stimulation of IgM secretion by products of a T cell hybridoma and a T cell line.
    J Immunol. 1981 Nov;127(5):1953-8 PMID: 6975305
  13. Replacement of T-cell function by a T-cell product.
    Nat New Biol. 1972 May 3;237(70):15-7 PMID: 4503682
  14. Evidence for two distinct classes of murine B cell growth factors with activities in different functional assays.
    J Exp Med. 1983 Sep 1;158(3):822-35 PMID: 6604127
  15. Induction of proliferation and Ig production in human B leukemic cells by anti-immunoglobulins and T cell factors.
    J Immunol. 1982 Mar;128(3):1296-301 PMID: 6799573
  16. Interleukin 1 and T cell activation.
    Immunol Rev. 1982;63:51-72 PMID: 6804369
  17. Role of BCGFII in the differentiation to antibody secretion normal and tumor B cells.
    J Immunol. 1985 Jun;134(6):3934-43 PMID: 2580899
  18. Role of gamma-interferon in antibody-producing responses.
    Nature. 1984 Jun 28-Jul 4;309(5971):799-801 PMID: 6429546
  19. Isolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities.
    Proc Natl Acad Sci U S A. 1986 Apr;83(7):2061-5 PMID: 3083412
  20. Factors controlling the B-cell cycle.
    Annu Rev Immunol. 1986;4:13-36 PMID: 2939855
  21. Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2).
    Proc Natl Acad Sci U S A. 1985 Aug;82(16):5490-4 PMID: 2410927
  22. Factors affecting B-cell growth and differentiation.
    Annu Rev Immunol. 1985;3:133-57 PMID: 3933529
  23. Activated B cells express receptors for, and proliferate in response to, pure interleukin 2.
    J Exp Med. 1984 Oct 1;160(4):1170-83 PMID: 6434689
  24. Antigen-induced T cell-replacing factor (TRF). III. Establishment of T cell hybrid clone continuously producing TRF and functional analysis of released TRF.
    J Immunol. 1980 Dec;125(6):2646-53 PMID: 6159416
  25. Identification of a T cell-derived b cell growth factor distinct from interleukin 2.
    J Exp Med. 1982 Mar 1;155(3):914-23 PMID: 6977612
  26. BCGFII activity on activated B cells of a purified murine T cell-replacing factor (TRF) from a T cell hybridoma (B151K12).
    J Immunol. 1985 Jun;134(6):3944-51 PMID: 3872907
  27. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes.
    Nature. 1985 Jun 20-26;315(6021):669-72 PMID: 3925347
  28. Both interleukin 2 and a second T cell-derived factor in EL-4 supernatant have activity as differentiation factors in IgM synthesis.
    J Exp Med. 1984 Dec 1;160(6):1605-21 PMID: 6439814
  29. Proliferation and differentiation of single hapten-specific B lymphocytes is promoted by T-cell factor(s) distinct from T-cell growth factor.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6350-4 PMID: 6983069
  30. Generation of cytotoxic T lymphocytes from thymocyte precursors to trinitrophenyl-modified self antigens. II. Establishment of a T cell hybrid clone constitutively producing killer-helper factor(s) (KHF) and functional analysis of released KHF.
    J Immunol. 1986 May 15;136(10):3553-60 PMID: 2939132
  31. Soluble factors involved in B cell differentiation: identification of two distinct T cell-replacing factors (TRF).
    J Immunol. 1983 May;130(5):2219-24 PMID: 6601148
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.13.4581
Published
1987-07-00
Pages
4581-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305134
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.13.4581 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2