Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Analytical biochemistry ·Vol. 162 ·No. 1 ·1987-04-00 ·Pages 156-9

Chomczynski P, Sacchi N

Abstract

A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.

MeSH Terms

Animals Cell Line Chloroform Guanidine Guanidines Humans Hydrogen-Ion Concentration Mammary Glands, Animal/analysis Phenol Phenols RNA/isolation & purification Rats Solutions

Chemicals

Guanidines Phenols Solutions Phenol RNA Chloroform Guanidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Chomczynski P

Sacchi N

Article Info

Journal

Analytical biochemistry

Abbr.

Anal Biochem

ISSN

0003-2697

DOI

10.1006/abio.1987.9999

Published

1987-04-00

Pages

156-9

Language

English

Region

United States

NLM ID

0370535

Subset

External Links

PubMed source DOI: 10.1006/abio.1987.9999 View journal details

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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

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