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PMID: 2440797 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibition of natural killer-cell mediated cytolysis with monoclonal antibodies to restricted and non-restricted epitopes of the leucocyte common antigen.

Immunology ·Vol. 61 ·No. 3 ·1987-07-00 ·Pages 351-6

Starling GC, Davidson SE, McKenzie JL, Hart DN

Abstract

Three monoclonal antibodies recognizing different epitopes of the leucocyte common molecule, CMRF-11 (against the restricted or B-220 leucocyte common molecule), CMRF-12 and CMRF-26 [each against a different epitope on the non-restricted or T200 leucocyte common (CD45) molecule], were tested for their effects on lymphocyte cytotoxicity. The individual monoclonal antibodies inhibited human natural killer cell-mediated cytolysis (NK-CMC) weakly, but a mixture of CMRF-11 + 12 + 26 antibodies inhibited cytolysis more consistently and to a greater extent. This mixture did not inhibit cytotoxic T lymphocytes derived from secondary mixed lymphocyte cultures. The CMRF-11 + 12 + 26 mix was shown to inhibit a post-conjugate formation stage of lysis at the effector cell level. Inhibition of NK-CMC of a wide range of target cells, including the T-cell lines Jurkat, HSB2 and Molt 4, was demonstrated.

MeSH Terms

Antibodies, Monoclonal Cytotoxicity, Immunologic Epitopes/immunology Histocompatibility Antigens/immunology Humans Killer Cells, Natural/immunology Leukocyte Common Antigens Lymphocyte Culture Test, Mixed T-Lymphocytes, Cytotoxic/immunology

Chemicals

Antibodies, Monoclonal Epitopes Histocompatibility Antigens Leukocyte Common Antigens

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Starling G C

Davidson S E

McKenzie J L

Hart D N

References (21)

21 references, click to expand

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Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1987-07-00

Pages

351-6

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1453401

Subset

External Links

PubMed source PMC Full Text View journal details

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Inhibition of natural killer-cell mediated cytolysis with monoclonal antibodies to restricted and non-restricted epitopes of the leucocyte common antigen.

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