Home LiteratureArticle Details
PMID: 24424698 Published · ppublish English Journal Article

The measurement of plant polyadenylic acid by hybridisation with radioactive polyuridylic acid.

Planta ·Vol. 131 ·No. 1 ·1976-01-00 ·Pages 75-9

Covey SN, Grierson D

Abstract

Saturation hybridisation of polyadenylic acid with [(3)H]polyuridylic acid is described. Under conditions of [(3)H]poly(U) excess, poly(A) is detected in the RNA of a number of higher plants. The ribonuclease resistant hybrids melt sharply when subjected to thermal denaturation. Plant RNA which contains poly(A) sequences detected by [(3)H]poly(U) hybridisation is polydisperse in molecular weight. Data presented shows that the amount of poly(A) in plant RNA is variable. This technique is useful for the qualitative and quantitative detection of poly(A) sequences in higher plant RNA.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Covey S N
Department of Physiology and Environmental Studies, University of Nottingham School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, UK.
Grierson D
References (15)
15 references, click to expand
  1. Isolation and in vitro translation of soybean leghaemoglobin mRNA.
    Nature. 1974 Sep 6;251(5470):74-7 PMID: 4414006
  2. Polyribosomes from peas: an improved method for their isolation in the absence of ribonuclease inhibitors.
    Plant Physiol. 1972 Nov;50(5):581-4 PMID: 16658221
  3. Polyadenylic acid sequences in yeast messenger ribonucleic acid.
    J Biol Chem. 1973 Feb 25;248(4):1466-71 PMID: 4568819
  4. Characterization and poly(adenylic acid) content of Ehrlich ascites cell ribonucleic acids fractionated on unmodified cellulose columns.
    Biochemistry. 1973 Jun 19;12(13):2395-403 PMID: 4350950
  5. Gibberellic acid causes increased synthesis of RNA which contains poly(A) in barley aleurone tissue.
    Proc Natl Acad Sci U S A. 1974 Aug;71(8):3290-3 PMID: 4528576
  6. The hybridisation of [(3)H]poly(uridylic) acid to DNA from Phaseolus aureus Roxb.
    Planta. 1975 Jan;127(1):87-91 PMID: 24430288
  7. The physical and chemical properties of nucleic acids.
    Annu Rev Biochem. 1967;36:407-48 PMID: 18257727
  8. Poly(A) sequences in plant polysomal RNA.
    Nat New Biol. 1973 Nov 21;246(151):68-70 PMID: 4519107
  9. Eukaryotic messenger RNA.
    Annu Rev Biochem. 1974;43(0):621-42 PMID: 4604447
  10. Poly(A)-associated RNA in plants.
    Biochim Biophys Acta. 1974 Apr 27;349(1):32-8 PMID: 11400435
  11. Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum.
    Nat New Biol. 1972 Oct 25;239(95):225-8 PMID: 4507801
  12. Subcellular distribution and properties of poly(A)-containing RNA from cultured plant cells.
    Eur J Biochem. 1976 Apr 1;63(2):599-606 PMID: 1261564
  13. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.
    Biochem J. 1969 Jun;113(1):131-8 PMID: 4896715
  14. Changes in the amount of ribosomal RNA and poly(A)-containing RNA during leaf development.
    Planta. 1975 Jan;127(1):77-86 PMID: 24430287
  15. Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid.
    J Mol Biol. 1974 May 5;85(1):75-86 PMID: 4835728
Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/BF00387348
Published
1976-01-00
Pages
75-9
Language
English
Region
Germany
NLM ID
1250576
External Links
PubMed source DOI: 10.1007/BF00387348 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2