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PMID: 2443911 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Heterologous enzyme function in Escherichia coli and the selection of genes encoding the catalytic RNA subunit of RNase P.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 19 ·1987-10-00 ·Pages 6825-9

Lawrence NP, Richman A, Amini R, Altman S

Abstract

The gene for the catalytic RNA subunit of RNase P has been isolated from several Enterobacteriaceae by complementation of an Escherichia coli strain that is temperature-sensitive for RNase P activity. The selection procedure relies on the ability of the heterologous gene products to function enzymatically in E. coli. This procedure obviates the need for positive results in DNA blot hybridization experiments or for the purification of holoenzyme to identify the RNA component of RNase P and its corresponding gene from organisms other than E. coli. Comparisons of the variations in sequences provide the basis for a refined two-dimensional model of the secondary structure of M1 RNA.

MeSH Terms
Base Sequence Endoribonucleases/genetics Escherichia coli/enzymology,genetics Escherichia coli Proteins Genes Genes, Bacterial Genetic Vectors Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial/genetics Ribonuclease P
Chemicals
Escherichia coli Proteins RNA, Bacterial Endoribonucleases Ribonuclease P ribonuclease P, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lawrence N P
Department of Biology, Yale University, New Haven, CT 06520.
Richman A
Amini R
Altman S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.19.6825
Published
1987-10-00
Pages
6825-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299177
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.19.6825 PMC Full Text View journal details
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