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PMID: 2445288 Published · ppublish English Journal Article

Development of a defined medium and two-step culturing method for improved exotoxin A yields from Pseudomonas aeruginosa.

Applied and environmental microbiology ·Vol. 53 ·No. 9 ·1987-09-00 ·Pages 2013-20

Blumentals II, Kelly RM, Gorziglia M, Kaufman JB, Shiloach J

Abstract

A two-step method is described for the production of exotoxin A by Pseudomonas aeruginosa in which a defined growth medium is modified for the toxin production phase. As a result, specific exotoxin A yields comparable to those obtained with complex media were achieved. In the development of this two-step process, several divalent metallic cations (Ca2+, Cu2+, and Mn2+), in addition to iron, were found to inhibit the yield of exotoxin A while Ca2+ and glycerol were found to increase yields. Northern blot analysis of total RNA isolates suggests that these effects on exotoxin A yields are based on events at the transcription level.

MeSH Terms
ADP Ribose Transferases Bacterial Toxins Calcium/pharmacology Cations, Divalent/pharmacology Copper/pharmacology Culture Media Exotoxins/biosynthesis,genetics Genes, Bacterial Glycerol/metabolism Immunoassay Iron/pharmacology Manganese/pharmacology Pseudomonas aeruginosa/drug effects,genetics,growth & development,metabolism RNA, Bacterial/analysis Transcription, Genetic/drug effects Virulence Factors
Chemicals
Bacterial Toxins Cations, Divalent Culture Media Exotoxins RNA, Bacterial Virulence Factors Manganese Copper Iron ADP Ribose Transferases toxA protein, Pseudomonas aeruginosa Glycerol Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Blumentals I I
Department of Chemical Engineering, Johns Hopkins University, Baltimore, Maryland 21218.
Kelly R M
Gorziglia M
Kaufman J B
Shiloach J
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.53.9.2013-2020.1987
Published
1987-09-00
Pages
2013-20
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC204050
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.53.9.2013-2020.1987 PMC Full Text View journal details
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