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PMID: 2445685 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization by affinity electrophoresis of an alpha-1,6-glucan-binding protein from Streptococcus sobrinus.

Infection and immunity ·Vol. 55 ·No. 12 ·1987-12-00 ·Pages 3011-6

Landale EC, McCabe MM

Abstract

Glucan-binding protein 1 (GBP1), the most abundant glucan-binding protein isolated from culture supernatants of Streptococcus sobrinus 6715-49, has been purified by affinity chromatography on Sephadex G-50 followed by gel permeation chromatography with Bio-Gel P-10. The specificity and affinity of GBP1 for glucans were assessed by affinity electrophoresis. GBP1 did not detectably bind to glucans lacking linear arrays of alpha-1,6 linkages. The association constant for the linear alpha-1,6-glucan Dextran T2000 was 3 x 10(7) M-1. Providing small isomaltosaccharide ligands to compete with this dextran indicated that the binding site maximally accommodated isomaltosaccharides with a degree of polymerization of 8. When glucans produced by purified S. sobrinus glucosyltransferases were tested, GBP1 displayed the highest affinity for the glucan from the soluble-product, primer-independent glucosyltransferase.

MeSH Terms
Bacterial Proteins/metabolism Carrier Proteins/metabolism Dextrans/metabolism Electrophoresis/methods Glucans/metabolism Isomaltose/metabolism Lectins Ligands Molecular Weight Streptococcus/analysis Structure-Activity Relationship
Chemicals
Bacterial Proteins Carrier Proteins Dextrans Glucans Lectins Ligands glucan-binding proteins Isomaltose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Landale E C
Department of Microbiology and Immunology, University of Miami School of Medicine, Florida 33101.
McCabe M M
References (22)
22 references, click to expand
  1. Dextran-induced agglutination of Streptococcus mutans, and its potential role in the formation of microbial dental plaques.
    J Bacteriol. 1969 May;98(2):341-6 PMID: 5784196
  2. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
  3. The binding of desialylated glycoproteins by plasma membranes of rat liver.
    J Biol Chem. 1971 Aug 10;246(15):4825-33 PMID: 4327329
  4. Multiphasic zone electrophoresis. 3. Further analysis and new forms of discontinuous buffer systems.
    Biochemistry. 1973 Feb 27;12(5):890-8 PMID: 4686806
  5. Binding properties of immunoglobulin combining sites specific for terminal or nonterminal antigenic determinants in dextran.
    J Exp Med. 1975 Aug 1;142(2):435-59 PMID: 49389
  6. Relationship between cell-bound dextransucrase and the agglutination of Streptococcus mutans.
    Infect Immun. 1975 Sep;12(3):512-20 PMID: 809356
  7. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.
    Anal Biochem. 1977 May 1;79(1-2):544-52 PMID: 68686
  8. Purification of dextran-binding protein from cariogenic Streptococcus mutans.
    Biochem Biophys Res Commun. 1977 Sep 9;78(1):273-8 PMID: 907676
  9. Aggregation of sponge cells. Function of a lectin in its homologous biological system.
    J Biol Chem. 1979 Aug 25;254(16):7479-81 PMID: 112094
  10. The role of bacterial surface structures in pathogenesis.
    Crit Rev Microbiol. 1981;8(4):303-38 PMID: 6115736
  11. Immunochemical characterization of binding sites of hybridoma antibodies specific for alpha (1 leads to 6) linked dextran.
    Mol Immunol. 1982 Mar;19(3):375-88 PMID: 6178961
  12. Association constants of hybridoma antibodies specific for alpha (1 leads to 6) linked dextran determined by affinity electrophoresis.
    Mol Immunol. 1982 Mar;19(3):389-97 PMID: 6178962
  13. Thermodynamics of binding between saccharides and Helix pomatia A hemagglutinin.
    Biochim Biophys Acta. 1983 Jan 12;742(1):235-42 PMID: 6824683
  14. Three kinds of extracellular glucosyltransferases from Streptococcus mutans 6715 (serotype g).
    FEBS Lett. 1983 Jun 27;157(1):79-84 PMID: 6222915
  15. Microbial exopolysaccharides -- their role in microbial adhesion in aqueous systems.
    Crit Rev Microbiol. 1983;10(2):173-201 PMID: 6354590
  16. Proteinaceous bacterial adhesins and their receptors.
    Crit Rev Microbiol. 1983;10(3):229-60 PMID: 6138214
  17. Soluble lectins: a new class of extracellular proteins.
    Science. 1984 Mar 23;223(4642):1259-64 PMID: 6367039
  18. Affinity electrophoresis.
    Methods Enzymol. 1984;104:275-81 PMID: 6717285
  19. Fimbrial adhesions of Escherichia coli.
    Rev Infect Dis. 1985 May-Jun;7(3):321-40 PMID: 2862689
  20. Purification and characterization of a primer-independent glucosyltransferase from Streptococcus mutans 6715-13 mutant 27.
    Infect Immun. 1985 Dec;50(3):771-7 PMID: 2933338
  21. Cell adhesion molecules in the regulation of animal form and tissue pattern.
    Annu Rev Cell Biol. 1986;2:81-116 PMID: 3548776
  22. Observations on the structure of pullulan.
    Arch Biochem Biophys. 1971 Mar;143(1):138-42 PMID: 4327233
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.12.3011-3016.1987
Published
1987-12-00
Pages
3011-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260021
Subset
IM
Grants
NIDCR NIH HHS · DE 04321 · United States
External Links
PubMed source DOI: 10.1128/iai.55.12.3011-3016.1987 PMC Full Text View journal details
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