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PMID: 2448792 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of the T-cell surface molecule CD2 and an epitope-loss CD2 mutant to define the role of lymphocyte function-associated antigen 3 (LFA-3) in T-cell activation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 4 ·1988-02-00 ·Pages 1194-8

Bierer BE, Peterson A, Barbosa J, Seed B, Burakoff SJ

Abstract

To define the role of the CD2-lymphocyte function-associated antigen 3 (LFA-3) interaction in T-cell activation, we have expressed a cDNA encoding the human CD2 molecule in a murine antigen-specific T-cell hybridoma. Expression of the CD2 molecule greatly enhances T-cell responsiveness to antigen; this enhancement is inhibited by anti-CD2 and anti-LFA-3 monoclonal antibodies (mAbs). CD2+ hybridomas produce interleukin 2 in response to combinations of anti-CD2 mAbs 9.6 and 9-1 and, in the presence of mAb 9-1, to sheep erythrocytes or to the LFA-3 antigen. Furthermore, hybridomas expressing a mutant CD2 molecule that has lost mAb 9.6 binding do not exhibit the enhanced response to antigen or the ability to respond to LFA-3 plus mAb 9-1, but these hybridomas retain the ability to respond to combinations of anti-CD2 mAbs. The role of the CD2-LFA-3 interaction in T-cell activation and the potential for other physiologic ligands for CD2 are discussed.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigens, Surface/immunology,physiology CD2 Antigens Carrier Proteins/biosynthesis,genetics,immunology Epitopes/genetics,immunology Erythrocytes/immunology Humans Hybridomas/immunology,metabolism Interleukin-2/biosynthesis Lymphocyte Activation Lymphocyte Function-Associated Antigen-1 Mice Mutation Receptors, Immunologic/biosynthesis,genetics,immunology Sheep T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Antigens, Surface CD2 Antigens Carrier Proteins Epitopes Interleukin-2 Lymphocyte Function-Associated Antigen-1 Receptors, Immunologic
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bierer B E
Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115.
Peterson A
Barbosa J
Seed B
Burakoff S J
References (31)
31 references, click to expand
  1. Monoclonal antibodies OKT 11 and OKT 11A have pan-T reactivity and block sheep erythrocyte "receptors".
    Eur J Immunol. 1982 Jan;12(1):81-6 PMID: 6977453
  2. Monoclonal antibody and ligand binding sites of the T cell erythrocyte receptor (CD2).
    Nature. 1987 Oct 29-Nov 4;329(6142):842-6 PMID: 2444890
  3. Alternative pathway activation of T cells by binding of CD2 to its cell-surface ligand.
    Nature. 1987 Mar 19-25;326(6110):298-301 PMID: 3102975
  4. Identification of a human T lymphocyte surface protein associated with the E-rosette receptor.
    J Exp Med. 1981 Jan 1;153(1):207-12 PMID: 6969782
  5. Two antigen-independent adhesion pathways used by human cytotoxic T-cell clones.
    Nature. 1986 Sep 18-24;323(6085):262-4 PMID: 3093887
  6. A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation.
    J Immunol. 1981 Jun;126(6):2117-22 PMID: 6785348
  7. T cell activation via CD2 [T, gp50] molecules: accessory cells are required to trigger T cell activation via CD2-D66 plus CD2-9.6/T11(1) epitopes.
    J Immunol. 1985 Sep;135(3):1624-31 PMID: 2410496
  8. T cell stimulation via the erythrocyte receptor. Synergism between monoclonal antibodies and phorbol myristate acetate without changes of free cytoplasmic Ca++ levels.
    J Exp Med. 1986 Mar 1;163(3):654-64 PMID: 2419470
  9. Expression and function of CD4 in a murine T-cell hybridoma.
    Nature. 1987 Jul 23-29;328(6128):351-3 PMID: 3037388
  10. Identification of a monoclonal antibody specific for a murine T3 polypeptide.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8 PMID: 2950524
  11. Rosette formation by peripheral lymphocytes.
    Clin Exp Immunol. 1970 May;6(5):681-8 PMID: 5477925
  12. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50.
    J Immunol. 1983 Jul;131(1):180-5 PMID: 6190907
  13. A common pathway for T lymphocyte activation involving both the CD3-Ti complex and CD2 sheep erythrocyte receptor determinants.
    J Immunol. 1986 Aug 15;137(4):1097-100 PMID: 2426350
  14. An alternative pathway of T-cell activation: a functional role for the 50 kd T11 sheep erythrocyte receptor protein.
    Cell. 1984 Apr;36(4):897-906 PMID: 6231105
  15. Thymocyte binding to human thymic epithelial cells is inhibited by monoclonal antibodies to CD-2 and LFA-3 antigens.
    J Immunol. 1987 Jan 15;138(2):358-63 PMID: 3098838
  16. T cell growth factor: parameters of production and a quantitative microassay for activity.
    J Immunol. 1978 Jun;120(6):2027-32 PMID: 307029
  17. Rosetting of activated human T lymphocytes with autologous erythrocytes. Definition of the receptor and ligand molecules as CD2 and lymphocyte function-associated antigen 3 (LFA-3).
    J Exp Med. 1987 Mar 1;165(3):664-76 PMID: 3102675
  18. Suppression of human T-cell mitogenesis and E-rosette formation by the monoclonal antibody OKT11A.
    Immunology. 1981 Dec;44(4):865-71 PMID: 6976312
  19. T cell rosette formation in primates, pigs, and guinea pigs. The influence of immunosuppresive agents.
    J Allergy Clin Immunol. 1974 Aug;54(2):86-93 PMID: 4546937
  20. LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function.
    Immunol Rev. 1982;68:171-95 PMID: 6184305
  21. Phenomenon of human T cells rosetting with sheep erythrocytes analyzed with monoclonal antibodies. "Modulation" of a partially hidden epitope determining the conditions of interaction between T cells and erythrocytes.
    J Exp Med. 1982 May 1;155(5):1317-33 PMID: 6175720
  22. Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion.
    J Exp Med. 1987 Mar 1;165(3):677-92 PMID: 3102676
  23. Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3365-9 PMID: 2437578
  24. Is the E receptor on human T lymphocytes a "negative signal receptor"?
    J Immunol. 1982 Dec;129(6):2479-85 PMID: 6815269
  25. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7489-93 PMID: 6984191
  26. In vitro rosette inhibition by antihuman antilymphocyte serum. Correlation with skin graft prolongation in subhuman primates.
    Transplantation. 1969 Sep;8(3):265-80 PMID: 4985394
  27. Human autologous rosette-forming cells. III. Binding of erythrocytes from different species to the T-cell receptors for autologous red blood cells.
    Cell Immunol. 1985 Nov;96(1):126-36 PMID: 3879720
  28. Rosetting of human red blood cells to thymocytes and thymus-derived cells.
    Clin Exp Immunol. 1973 Nov;15(3):385-92 PMID: 4202949
  29. Direct evidence for a receptor-ligand interaction between the T-cell surface antigen CD2 and lymphocyte-function-associated antigen 3.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6864-8 PMID: 3309949
  30. The functional significance, distribution, and structure of LFA-1, LFA-2, and LFA-3: cell surface antigens associated with CTL-target interactions.
    J Immunol. 1983 Aug;131(2):611-6 PMID: 6345670
  31. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.
    J Immunol. 1985 Jun;134(6):3994-4000 PMID: 2580901
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.4.1194
Published
1988-02-00
Pages
1194-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279733
Subset
IM
Grants
NCI NIH HHS · CA 34129 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.4.1194 PMC Full Text View journal details
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