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PMID: 2455724 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A growth factor-repressible gene associated with protein kinase C-mediated inhibition of adipocyte differentiation.

The Journal of cell biology ·Vol. 107 ·No. 1 ·1988-07-00 ·Pages 279-86

Navre M, Ringold GM

Abstract

The conversion of determined adipoblasts to fully differentiated adipocytes requires appropriate environmental conditions. A strict dependence on cell confluence and a facilitation by glucocorticoid hormones have previously been described. We have found that agents that are capable of activating protein kinase C, such as basic fibroblast growth factor and phorbol esters, inhibit the differentiation of the adipogenic cell line TA1 without stimulating cell growth. Here we describe the sequence and characterization of a cDNA (clone 5) that detects an RNA, the expression of which is enhanced by glucocorticoids and increasing cell density. In contrast, activators of protein kinase C including basic fibroblast growth factor, phorbol esters, and synthetic diacylglycerols inhibit clone 5 gene expression. It appears that clone 5 expression is closely linked to environmental and hormonal factors that promote the differentiation of adipogenic cells.

MeSH Terms
Adipose Tissue/cytology Amino Acid Sequence Base Sequence Cell Differentiation/drug effects Cell Line Cloning, Molecular Culture Media DNA/genetics Enzyme Activation Fibroblast Growth Factors/pharmacology Gene Expression Regulation/drug effects Molecular Sequence Data Protein Kinase C/metabolism RNA/genetics Sequence Homology, Nucleic Acid
Chemicals
Culture Media Fibroblast Growth Factors RNA DNA Protein Kinase C
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Navre M
Department of Pharmacology, Stanford University School of Medicine, California 94305.
Ringold G M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.1.279
Published
1988-07-00
Pages
279-86
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115191
Subset
IM
Grants
NIGMS NIH HHS · GM 25821 · United States
Databases
GENBANK
X07411
External Links
PubMed source DOI: 10.1083/jcb.107.1.279 PMC Full Text View journal details
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