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PMID: 2455893 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Quantitative analysis of RNA produced by slow and fast alleles of Adh in Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 14 ·1988-07-00 ·Pages 5161-5

Laurie CC, Stam LF

Abstract

The alcohol dehydrogenase (ADH) locus (Adh) of Drosophila melanogaster in polymorphic on a world-wide basis for two allozymes, Fast and Slow. This study was undertaken to determine whether the well-established difference in ADH protein concentration between the allozymes is due to a difference in mRNA levels. RNA gel blot hybridization and an RNase protection assay were used to quantify ADH mRNA levels. Each method used an Adh null mutant as an internal standard. Several Slow and Fast allele pairs of different geographic origins were analyzed. The results provide strong evidence that the ADH protein concentration difference is not accounted for by RNA level.

MeSH Terms
Alcohol Dehydrogenase/genetics Alleles Animals Drosophila melanogaster/genetics Heterozygote Mutation Nucleic Acid Hybridization Polymorphism, Genetic RNA/biosynthesis RNA, Messenger/biosynthesis Ribonucleases/metabolism Transformation, Genetic
Chemicals
RNA, Messenger RNA Alcohol Dehydrogenase Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Laurie C C
Department of Genetics, North Carolina State University, Raleigh 27695.
Stam L F
References (25)
25 references, click to expand
  1. Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster.
    Genetics. 1987 Jan;115(1):129-40 PMID: 2881843
  2. Deletions at intervening sequence splice sites in the alcohol dehydrogenase gene of Drosophila.
    Nucleic Acids Res. 1982 Nov 25;10(22):7261-72 PMID: 6296769
  3. The relative quantities and catalytic activities of enzymes produced by alleles at the alcohol dehydrogenase locus in Drosophila melanogaster.
    Biochem Genet. 1974 Feb;11(2):155-65 PMID: 4207494
  4. Genetic control of alcohol dehydrogenase levels in Drosophila.
    Biochem Genet. 1978 Jun;16(5-6):509-23 PMID: 104709
  5. The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5' end in different developmental stages.
    Cell. 1983 May;33(1):125-33 PMID: 6432335
  6. The alcohol dehydrogenase alleloenzymes AdhS and AdhF from the fruitfly Drosophila melanogaster: an enzymatic rate assay to determine the active-site concentration.
    Biochem Genet. 1985 Apr;23(3-4):205-16 PMID: 3160338
  7. Biochemical differences between products of the Adh locus in Drosophila.
    Genetics. 1980 Aug;95(4):1013-22 PMID: 6781982
  8. Immunochemical quantitation of antigens by single radial immunodiffusion.
    Immunochemistry. 1965 Sep;2(3):235-54 PMID: 4956917
  9. Formaldehyde mutagenesis in Drosophila. Molecular analysis of ADH-negative mutants.
    Mutat Res. 1983 Sep;111(1):1-7 PMID: 6413850
  10. Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the Drosophila germ line.
    Cell. 1983 Aug;34(1):59-73 PMID: 6309412
  11. Nucleotide polymorphism at the alcohol dehydrogenase locus of Drosophila melanogaster.
    Nature. 1983 Aug 4-10;304(5925):412-7 PMID: 6410283
  12. Structural analysis of the ADHS electromorph of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5609-12 PMID: 103098
  13. Structure and transcription of the Drosophila mulleri alcohol dehydrogenase genes.
    Nucleic Acids Res. 1985 Oct 11;13(19):6899-917 PMID: 2997730
  14. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  15. Genetic and biochemical basis of enzyme activity variation in natural populations. I. Alcohol dehydrogenase in Drosophila melanogaster.
    Genetics. 1978 Jun;89(2):371-88 PMID: 97168
  16. Genetical variation for enzyme activity in a population of Drosophila melanogaster. VI. Molecular variation in the control of alcohol dehydrogenase (ADH) activity.
    Heredity (Edinb). 1981 Oct;47(2):185-96 PMID: 6797989
  17. Genetic variation in the expression of ADH in Drosophila melanogaster.
    Genetics. 1982 Jul-Aug;101(3-4):431-46 PMID: 6816669
  18. Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1073-7 PMID: 16592769
  19. Variation in amount of enzyme protein in natural populations.
    Biochem Genet. 1978 Apr;16(3-4):159-70 PMID: 98165
  20. Biochemical and molecular analysis of naturally occurring Adh variants in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4798-802 PMID: 6410397
  21. Mutation of the Adh gene of Drosophila melanogaster containing an internal tandem duplication.
    J Mol Biol. 1985 Dec 20;186(4):679-88 PMID: 2419573
  22. Molecular population genetics of the alcohol dehydrogenase gene region of Drosophila melanogaster.
    Genetics. 1986 Dec;114(4):1165-90 PMID: 3026893
  23. Differences in the number of molecules produced by two allelic electrophoretic enzyme variants in D. melanogaster.
    Experientia. 1972 Aug 15;28(8):975-6 PMID: 4627695
  24. Molecular analysis of X-ray-induced alcohol dehydrogenase (ADH) null mutations in Drosophila melanogaster.
    Genetics. 1985 Feb;109(2):365-77 PMID: 2982699
  25. Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes.
    Science. 1985 Dec 13;230(4731):1242-6 PMID: 4071043
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.14.5161
Published
1988-07-00
Pages
5161-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281708
Subset
IM
Grants
NIGMS NIH HHS · GM11546 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.14.5161 PMC Full Text View journal details
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