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PMID: 2457904 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 16 ·1988-08-00 ·Pages 5951-5

Gruenert DC, Basbaum CB, Welsh MJ, Li M, Finkbeiner WE, Nadel JA

Abstract

To facilitate understanding of the mechanisms underlying pulmonary diseases, including lung cancer and cystic fibrosis, we have transformed and characterized cultures of human tracheal epithelial cells. Cells were transfected by calcium phosphate precipitation with a plasmid containing a replication-defective simian virus 40 (SV40) genome. Colonies of cells with enhanced growth potential were isolated and analyzed for transformation- and epithelial-specific characteristics. Precrisis cells were observed to express the SV40 large tumor antigen, produce cytokeratins, have microvilli, and form tight junctions. After crisis, cells continued to express the SV40 large tumor antigen as well as epithelial-specific cytokeratins and to display the apical membrane microvilli. Apical membrane Cl channels were opened in postcrisis cells exposed to 50 microM forskolin. These channels showed electrical properties similar to those observed in primary cultures. The postcrisis cells have been in culture for greater than 250 generations and are potentially "immortal." In addition to providing a useful in vitro model for the study of ion transport by human airway epithelial cells, the cells can be used to examine stages of neoplastic progression.

MeSH Terms
Cell Line, Transformed Cell Transformation, Neoplastic Cell Transformation, Viral Chlorides/metabolism Defective Viruses Epithelium/metabolism,pathology Humans Ion Channels/physiology Keratins/analysis Oncogenes Simian virus 40 Trachea/metabolism,pathology
Chemicals
Chlorides Ion Channels Keratins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gruenert D C
Cardiovascular Research Institute, University of California, San Francisco 94143.
Basbaum C B
Welsh M J
Li M
Finkbeiner W E
Nadel J A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.16.5951
Published
1988-08-00
Pages
5951-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281883
Subset
IM
Grants
NHLBI NIH HHS · HL 29851 · United States
NHLBI NIH HHS · HL24136 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.16.5951 PMC Full Text View journal details
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