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PMID: 2457913 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Malignant transformation of mouse primary keratinocytes by Harvey sarcoma virus and its modulation by surrounding normal cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 17 ·1988-09-00 ·Pages 6389-93

Dotto GP, Weinberg RA, Ariza A

Abstract

The activated ras oncogene that is present in Harvey sarcoma virus is able to induce malignant transformation of pure cultures of mouse primary keratinocytes. Malignant transformation of these cells is demonstrated by their ability to form carcinomas when grafted back onto syngeneic animals. However, expression of the malignant phenotype by the ras-transformed keratinocytes is drastically inhibited by the presence of normal dermal fibroblasts. This inhibitory effect depends on the ratio of fibroblasts to keratinocytes. It can be observed with mitomycin C-treated growth-arrested dermal fibroblasts and not with other cells, such as normal keratinocytes or established fibroblasts. Thus, a cellular environment approximating normal tissue can suppress tumor formation triggered by a single oncogene.

MeSH Terms
Animals Animals, Newborn Cell Division Cell Transformation, Neoplastic Cells, Cultured Epidermal Cells Epidermis/transplantation Fibroblasts/cytology Genes, ras Harvey murine sarcoma virus/genetics Keratins/biosynthesis Mice Mice, Inbred BALB C Sarcoma Viruses, Murine/genetics Skin/cytology
Chemicals
Keratins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dotto G P
Department of Pathology, Yale University School of Medicine, New Haven, CT 06510.
Weinberg R A
Ariza A
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.17.6389
Published
1988-09-00
Pages
6389-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281977
Subset
IM
Grants
NCI NIH HHS · CA39826 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.17.6389 PMC Full Text View journal details
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