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PMID: 2457916 Published · ppublish English Journal Article

cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 18 ·1988-09-00 ·Pages 6706-10

Ladner MB, Martin GA, Noble JA, Wittman VP, Warren MK, McGrogan M, Stanley ER

Abstract

A 4-kilobase and a 2-kilobase cDNA clone encoding a murine macrophage colony-stimulating factor have been isolated. Except for 2 amino acid residue differences, these two clones encode the same 520 amino acid residue protein, which is preceded by a 32-amino acid residue signal peptide. The two clones, whose molecular masses correspond to the two transcripts observed in murine L929 fibroblasts, contain 3' untranslated regions that are markedly different in sequence and length. Both clones can be expressed in COS cells and the recombinant protein is active in a mouse bone marrow colony assay.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Colony-Stimulating Factors/genetics DNA/analysis Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Granulocyte-Macrophage Colony-Stimulating Factor Growth Substances/genetics Mice Molecular Sequence Data RNA/analysis
Chemicals
Colony-Stimulating Factors Growth Substances RNA Granulocyte-Macrophage Colony-Stimulating Factor DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ladner M B
Department of Molecular Biology, Cetus Corp., Emeryville, CA 94608.
Martin G A
Noble J A
Wittman V P
Warren M K
McGrogan M
Stanley E R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.18.6706
Published
1988-09-00
Pages
6706-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282046
Subset
IM
Databases
GENBANK
J03862, M21149, M21952
External Links
PubMed source DOI: 10.1073/pnas.85.18.6706 PMC Full Text View journal details
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