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PMID: 2458428 Published · ppublish English Journal Article

Hydrogen ion modulation of Ca channel current in cardiac ventricular cells. Evidence for multiple mechanisms.

The Journal of general physiology ·Vol. 91 ·No. 5 ·1988-05-00 ·Pages 641-57

Krafte DS, Kass RS

Abstract

We have investigated the effects of H ions on (L-type) Ca channel current in isolated ventricular cells. We find that the current amplitude is enhanced in solutions that are alkaline relative to pH 7.4 and reduced in solutions acidic to this pH. We measured pH0-induced shifts in channel gating and analyzed our results in terms of surface potential theory. The shifts are well described by changes in surface potential caused by the binding of H ions to negative charges on the cell surface. The theory predicts a pK of 5.8 for this binding. Gating shifts alone cannot explain all of our observations on modulation of current amplitude. Our results suggest that an additional mechanism contributes to modification of the current amplitude.

MeSH Terms
Animals Calcium/metabolism Electric Conductivity Female Guinea Pigs Hydrogen-Ion Concentration Ion Channels/metabolism Male Membrane Potentials Myocardium/cytology,metabolism Protons Rats Rats, Inbred Strains
Chemicals
Ion Channels Protons Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Krafte D S
Department of Physiology, University of Rochester, New York 14642.
Kass R S
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.91.5.641
Published
1988-05-00
Pages
641-57
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2216148
Subset
IM
External Links
PubMed source DOI: 10.1085/jgp.91.5.641 PMC Full Text View journal details
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