Home LiteratureArticle Details

PMID: 2460092 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification and characterization of an endogenous inhibitor of the sialyltransferase CMP-N-acetylneuraminate: lactosylceramide alpha 2,6-N-acetylneuraminyltransferase (EC 2.4.99.-).

The Biochemical journal ·Vol. 254 ·No. 2 ·1988-09-01 ·Pages 559-65

Albarracin I, Lassaga FE, Caputto R

Abstract

The presence in the 100,000 g supernatant of rat brain homogenate of an inhibitor of the sialyltransferase has been confirmed. It is also present in chicken and bovine brain and in other rat and bovine organs. The inhibitor has been purified, a preparation with a specific activity 130-fold higher than that of the original 100,000 g supernatant of brain being obtained. It runs as a single peak in polyacrylamide-gel electrophoresis; when run in the presence of SDS, two components appeared. The apparent Mr of the components were 14,800 and 22,400. The inhibitor has been characterized as a heat-stable protein of acidic nature. It has effect on the glycolipid and the glycoprotein sialyltransferase activities but has no effect on the galactosaminyltransferase activity.

MeSH Terms

Amino Acids/analysis Animals Asialoglycoproteins Brain/enzymology Cattle Chickens Chromatography, Gel Chromatography, Ion Exchange Chymotrypsin/pharmacology Fetuins Kinetics Molecular Weight Rats Sialyltransferases/antagonists & inhibitors Species Specificity Temperature Tissue Distribution Trypsin/pharmacology alpha-Fetoproteins/pharmacology

Chemicals

Amino Acids Asialoglycoproteins Fetuins alpha-Fetoproteins asialofetuin Sialyltransferases beta-D-galactoside alpha 2-6-sialyltransferase Chymotrypsin Trypsin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Albarracin I

Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.

Lassaga F E

Caputto R

References (24)

24 references, click to expand

The pattern of mammalian brain gangliosides. 3. Regional and developmental differences.
J Neurochem. 1965 Dec;12(12):969-79 PMID: 5892497
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
Synthesis of gangliosides during development and its relation to the quantitative changes of subcellular particles of rat brain.
J Neurochem. 1968 Nov;15(11):1257-64 PMID: 5707416
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Developmental profiles of gangliosides in human and rat brain.
J Neurochem. 1971 Apr;18(4):581-92 PMID: 5581573
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
A natural inhibitor of sialyl transferase and its possible influence on this enzyme activity during brain development.
Biochemistry. 1972 Apr 11;11(8):1396-400 PMID: 5021596
Galactosyltransferases catalyzing the formation of the galactosyl-galactosyl linkage in glycosphingolipids.
J Biol Chem. 1974 Jul 10;249(13):4132-7 PMID: 4852121
Regulation of rat-liver glycoprotein: N-acetylneuraminic acid transferase activity by pyrimidine nucleotides.
Eur J Biochem. 1975 Oct 15;58(2):477-81 PMID: 1183447
Effect of development on the gangliosides of human brain.
J Neurochem. 1977 Jun;28(6):1299-304 PMID: 874491
Improved manual sequencing methods.
Methods Enzymol. 1977;47:335-57 PMID: 927191
Isolation and partial characterization of an endogenous inhibitor of ceramide glycosyltransferases from rat brain.
J Biol Chem. 1978 Jan 25;253(2):340-2 PMID: 618873
Sialyltransferases in rat brain: reaction kinetics, product analyses, and multiplicities of enzyme species.
J Neurochem. 1977 Dec;29(6):1075-83 PMID: 74403
The biosynthesis of brain gangliosides. Separation of membranes with different ratios of ganglioside sialylating activity to gangliosides.
Biochem J. 1977 Dec 15;168(3):325-32 PMID: 606237
Complete amino acid sequence of rabbit beta 2-microglobulin.
Biochemistry. 1979 May 29;18(11):2267-72 PMID: 87220
Ganglioside patterns of fetal rat and mouse brain.
J Neurochem. 1980 Jun;34(6):1527-30 PMID: 7381476
Inhibition of the chicken retinal UDP-GaINAc:GM3, N-acetylgalactosaminyl-transferase by blood serum and by pineal gland extracts.
J Neurosci Res. 1984;12(2-3):269-76 PMID: 6438348
Synaptosomal sialyltransferase glycosylates surface proteins that are inaccessible to the action of membrane-bound sialidase.
J Neurochem. 1986 Oct;47(4):1176-80 PMID: 3746299
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Phosphorus assay in column chromatography.
J Biol Chem. 1959 Mar;234(3):466-8 PMID: 13641241
CHROMATOGRAPHIC SEPARATION OF HUMAN BRAIN GANGLIOSIDES.
J Neurochem. 1963 Sep;10:613-23 PMID: 14066623
INCORPORATION OF RADIOACTIVITY OF D-GLUCOSAMINE-1-C14,D-GLUCOSE-1-C14,D-GALACTOSE-1-C14, AND DL-SERINE-3-C14 INTO RAT BRAIN GLYCOLIPIDS.
Biochemistry. 1963 May-Jun;2:580-5 PMID: 14069552
Composition of phospholipids and of phospholipid fatty acids and aldehydes in human red cells.
J Lipid Res. 1967 Nov;8(6):667-75 PMID: 6057495

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2540559

Published

1988-09-01

Pages

559-65

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1135114

Subset

External Links

PubMed source DOI: 10.1042/bj2540559 PMC Full Text View journal details

Analysis Services

Purification and characterization of an endogenous inhibitor of the sialyltransferase CMP-N-acetylneuraminate: lactosylceramide alpha 2,6-N-acetylneuraminyltransferase (EC 2.4.99.-).

Contact

WeChat Official Account