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PMID: 2462926 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sarcoballs: direct access to sarcoplasmic reticulum Ca2+-channels in skinned frog muscle fibers.

Biophysical journal ·Vol. 54 ·No. 2 ·1988-08-00 ·Pages 357-63

Stein P, Palade P

Abstract

In skeletal muscle, twitch contraction is caused by the rapid release of Ca2+ from the sarcoplasmic reticulum (SR) (Endo, M. 1977. Physiol. Rev. 57:71-108) via Ca2+ conducting channels in the SR membrane (Smith, J. S., R. Coronado, and G. Meissner, 1985. Nature (Lond.). 316:446-449; Suarez-Isla, B. A., G. Orozco, P. F. Heller, and J. P. Froehlich. 1986. Proc. Natl. Acad. Sci. USA. 83:7741-7745). To facilitate study of these and other intracellular channels, we have developed a method which allows direct patch-clamp recording of currents through SR channels in native membrane. The Ca2+-release channel studied using this method exhibits two predominant conductance levels (80-100 pS and 120-160 pS), conducts Ca2+ preferentially over K+ (PCa/Pk = 6.5), is highly voltage sensitive, blocked on one side by ruthenium red (1 microM), and displays enhanced activity in the presence of caffeine (5 mM). Studied in skinned fibers, this channel appears fundamentally similar to homologous channels from isolated rabbit SR incorporated into bilayers, with some distinct differences.

MeSH Terms
Animals Calcium/metabolism Electric Conductivity In Vitro Techniques Ion Channels/physiology Membrane Potentials Microscopy, Electron Muscles/physiology Rana catesbeiana Sarcoplasmic Reticulum/physiology,ultrastructure
Chemicals
Ion Channels Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stein P
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77550.
Palade P
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(88)82967-9
Published
1988-08-00
Pages
357-63
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1330304
Subset
IM
Grants
NHLBI NIH HHS · 1PO1 HL37044 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(88)82967-9 PMC Full Text View journal details
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