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PMID: 2463378 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibition of human T-cell leukemia virus type I replication in primary human T cells that express antisense RNA.

Journal of virology ·Vol. 63 ·No. 2 ·1989-02-00 ·Pages 677-82

von Rüden T, Gilboa E

Abstract

The human T-cell leukemia virus type I is associated with adult T-cell leukemia-lymphoma in humans, a disease which is induced by a malignant transformation of T lymphocytes. Retrovirus vectors carrying human T-cell leukemia virus type I-derived sequences in reversed transcriptional orientation were used to express antisense RNA transcripts in primary human leukocytes. Human T-cell leukemia virus type I replication and virus-mediated immortalization were inhibited in cells harboring antisense constructs. This study suggests that retrovirus-mediated antisense RNA inhibition can be used to protect primary human T-lymphocytes from human T-cell leukemia virus type I-mediated cell transformation.

MeSH Terms
Cell Division Cell Line DNA Replication Gene Expression Regulation Genetic Vectors Human T-lymphotropic virus 1/genetics,physiology Humans RNA/genetics RNA, Antisense T-Lymphocytes/microbiology Virus Replication
Chemicals
RNA, Antisense RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
von Rüden T
Institute of Genetics, Johannes Gutenberg University of Mainz, Federal Republic of Germany.
Gilboa E
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36 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.2.677-682.1989
Published
1989-02-00
Pages
677-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247738
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.2.677-682.1989 PMC Full Text View journal details
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