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PMID: 2464577 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotidylation, not phosphorylation, is the major source of the phosphotyrosine detected in enteric bacteria.

Journal of bacteriology ·Vol. 171 ·No. 1 ·1989-01-00 ·Pages 272-9

Foster R, Thorner J, Martin GS

Abstract

The majority of the phosphotyrosine recovered from partial acid hydrolysates of 32P-labeled Escherichia coli is derived from a single prominent protein. We show here by biochemical, genetic, and immunological criteria that this protein is actually glutamine synthetase adenylylated (not phosphorylated) at tyrosine. Furthermore, all of the phosphotyrosine detectable in partial acid hydrolysates of 32P-labeled Salmonella typhimurium was eliminated in a strain deficient in both glutamine synthetase and uridylyltransferase, an enzyme which uridylylates the regulatory protein PII at a tyrosine residue. These results suggest that protein-tyrosine phosphorylation represents a rare modification in eubacterial cells.

MeSH Terms
Electrophoresis, Gel, Two-Dimensional/methods Escherichia coli/genetics,metabolism Glutamate-Ammonia Ligase/genetics,isolation & purification,metabolism Hydrolysis Molecular Weight Phosphates/metabolism Phosphorus Radioisotopes Phosphorylation Phosphoserine/isolation & purification Phosphothreonine/isolation & purification Phosphotyrosine Radioisotope Dilution Technique Salmonella typhimurium/genetics,metabolism Species Specificity Tyrosine/analogs & derivatives,biosynthesis,isolation & purification
Chemicals
Phosphates Phosphorus Radioisotopes Phosphothreonine Phosphoserine Phosphotyrosine Tyrosine Glutamate-Ammonia Ligase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Foster R
Department of Biochemistry, University of California, Berkeley 94720.
Thorner J
Martin G S
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40 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.1.272-279.1989
Published
1989-01-00
Pages
272-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209582
Subset
IM
Grants
NCI NIH HHS · CA09041 · United States
NIGMS NIH HHS · GM07232 · United States
NIGMS NIH HHS · GM21841 · United States
External Links
PubMed source DOI: 10.1128/jb.171.1.272-279.1989 PMC Full Text View journal details
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