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PMID: 2465298 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vitro angiogenesis on the human amniotic membrane: requirement for basic fibroblast growth factor-induced proteinases.

The Journal of cell biology ·Vol. 108 ·No. 2 ·1989-02-00 ·Pages 671-82

Mignatti P, Tsuboi R, Robbins E, Rifkin DB

Abstract

The role of basic fibroblast growth factor-(bFGF) induced proteinases in basement membrane (BM) invasion by bovine capillary endothelial (BCE) cells was studied using a quantitative in vitro assay previously described (Mignatti et al., 1986). 125I-iododeoxyuridine-labeled BCE cells were grown for 72 h on the human amnion BM, and cell invasion was determined by measuring the radioactivity associated with the tissue after removal of the noninvasive cell layer. BCE cells were noninvasive under normal conditions. Addition of human bFGF to either the BM or to the stromal aspect of the amnion induced BCE cell invasion with a dose-dependent response. This effect was maximal in the presence of 70 ng/ml bFGF, and was inhibited by anti-FGF antibody. Transforming growth factor beta, as well as plasmin inhibitors and anti-tissue type plasminogen activator antibody inhibited BCE cell invasion. The tissue inhibitor of metalloproteinases, 1-10 phenanthroline, anti-type IV and anti-interstitial collagenase antibodies had the same effect. On the contrary, anti-stromelysin antibody and Eglin, an inhibitor of elastase, were ineffective. The results obtained show that both the plasminogen activator-plasmin system and specific collagenases are involved in the invasive process occurring during angiogenesis.

MeSH Terms
Amnion/blood supply,cytology Animals Basement Membrane/blood supply,cytology Cattle Cell Adhesion Endopeptidases/metabolism Endothelium, Vascular/cytology Fibrinolysin/antagonists & inhibitors Fibroblast Growth Factors/pharmacology Humans Idoxuridine/metabolism Metalloendopeptidases/antagonists & inhibitors Neovascularization, Pathologic Protease Inhibitors/pharmacology Transforming Growth Factors/pharmacology
Chemicals
Protease Inhibitors Fibroblast Growth Factors Transforming Growth Factors Endopeptidases Fibrinolysin Metalloendopeptidases Idoxuridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mignatti P
Department of Cell Biology, New York University School of Medicine, New York 10016.
Tsuboi R
Robbins E
Rifkin D B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.2.671
Published
1989-02-00
Pages
671-82
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115414
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.108.2.671 PMC Full Text View journal details
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