Home LiteratureArticle Details
PMID: 2465483 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Positioning ribosomes on leader mRNA for translational activation of the message of an inducible Staphylococcus aureus cat gene.

Molecular & general genetics : MGG ·Vol. 214 ·No. 1 ·1988-09-00 ·Pages 108-11

Dick T, Matzura H

Abstract

The expression of the chloramphenicol (Cm) - inducible Cm acetyltransferase gene (cat) of the staphylococcal plasmid pUB112 is regulated at the translational level. The leader mRNA preceding the cat coding sequence can form a stable hairpin structure, in which the cat Shine-Dalgarno sequence is masked. Previous work showed that translation of a short leader peptide terminating within the stem of the inhibitory secondary structure is required for basal Cm acetyltransferase (CAT) synthesis and its inducibility. In the present study we shortened this leader peptide by introducing ochre codons in its coding sequence and found that synthesis of the N-terminal part of the leader peptide, terminating directly 5' to the stem, is sufficient to mediate basal and inducible CAT synthesis. Amino acid substitution in this region of the leader peptide abolished inducibility. We suggest that the 5' region of the leader peptide coding sequence specifies a particularly Cm-sensitive translation that represents the Cm-sensor mechanism for cat gene induction.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Base Sequence Chloramphenicol O-Acetyltransferase/genetics Codon Genes, Bacterial Molecular Sequence Data Mutation Protein Biosynthesis RNA, Bacterial/genetics,ultrastructure RNA, Messenger/genetics,ultrastructure Regulatory Sequences, Nucleic Acid Ribosomes/physiology Staphylococcus aureus/genetics Transformation, Genetic
Chemicals
Codon RNA, Bacterial RNA, Messenger Chloramphenicol O-Acetyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dick T
Molekulare Genetik der Universität, Heidelberg, Federal Republic of Germany.
Matzura H
References (20)
20 references, click to expand
  1. Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis.
    Nucleic Acids Res. 1986 Dec 22;14(24):9679-98 PMID: 3027659
  2. Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.
    Methods Enzymol. 1983;100:468-500 PMID: 6225933
  3. Chloramphenicol acetyltransferase: enzymology and molecular biology.
    CRC Crit Rev Biochem. 1983;14(1):1-46 PMID: 6340955
  4. S1 mapping of the pC194 encoded chloramphenicol acetyltransferase gene in Bacillus subtilis.
    Nucleic Acids Res. 1988 Feb 25;16(4):1618 PMID: 2831504
  5. Demonstration of erythromycin-dependent stalling of ribosomes on the ermC leader transcript.
    J Biol Chem. 1987 Feb 5;262(4):1766-71 PMID: 3027099
  6. A new mutator strain of Bacillus subtilis.
    Mol Gen Genet. 1987 Jun;208(1-2):353-6 PMID: 3112525
  7. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  8. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  9. Inhibition of ribosomal peptidyltransferase by chloramphenicol. Kinetic studies.
    Eur J Biochem. 1987 Apr 1;164(1):53-8 PMID: 3549307
  10. Translational attenuation: the regulation of bacterial resistance to the macrolide-lincosamide-streptogramin B antibiotics.
    CRC Crit Rev Biochem. 1984;16(2):103-32 PMID: 6203682
  11. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.
    Methods Enzymol. 1975;43:737-55 PMID: 1094240
  12. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  13. Construction and properties of chimeric plasmids in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1428-32 PMID: 418412
  14. Chloramphenicol-induced translation of cat-86 mRNA requires two cis-acting regulatory regions.
    J Bacteriol. 1985 Nov;164(2):696-703 PMID: 2414270
  15. Expression of a chloramphenicol-resistance determinant carried on hybrid plasmids in gram-positive and gram-negative bacteria.
    Gene. 1984 Dec;32(1-2):151-60 PMID: 6442250
  16. Chloramphenicol induces translation of the mRNA for a chloramphenicol-resistance gene in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3939-43 PMID: 3086871
  17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  18. Drug-free induction of a chloramphenicol acetyltransferase gene in Bacillus subtilis by stalling ribosomes in a regulatory leader.
    J Bacteriol. 1987 Sep;169(9):4235-41 PMID: 3114238
  19. Dependence of expression of an inducible Staphylococcus aureus cat gene on the translation of its leader sequence.
    Mol Gen Genet. 1987 May;207(2-3):486-91 PMID: 3112518
  20. Regulation of the inducible chloramphenicol acetyltransferase gene of the Staphylococcus aureus plasmid pUB112.
    EMBO J. 1985 Sep;4(9):2295-300 PMID: 3865770
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00340187
Published
1988-09-00
Pages
108-11
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00340187
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2