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PMID: 2468482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism.

Electrophoresis ·Vol. 10 ·No. 3 ·1989-03-00 ·Pages 186-9

Picard-Pasquier N, Ouagued M, Picard B, Goullet P, Krishnamoorthy R

Abstract

A rapid DNA extraction procedure and random primer labelling of Escherichia coli ribosomal RNA with cloned reverse transcriptase have been used to establish a simple and highly sensitive method for studying ribosomal DNA polymorphism in bacteria. Examples of inter- and intraspecies differentiation of bacteria are given and potential applications in bacterial epidemiology and taxonomy are discussed.

MeSH Terms

Acinetobacter/genetics Autoradiography Bacteria/genetics DNA, Bacterial/analysis DNA, Ribosomal/analysis Enterobacteriaceae/genetics Ethidium Methods Polymorphism, Genetic Polymorphism, Restriction Fragment Length RNA Probes RNA-Directed DNA Polymerase Species Specificity

Chemicals

DNA, Bacterial DNA, Ribosomal RNA Probes RNA-Directed DNA Polymerase Ethidium

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Picard-Pasquier N

Institut de Pathologie Moléculaire, Institut National de la Santé et de la Recherche Médicale, Paris, France.

Ouagued M

Picard B

Goullet P

Krishnamoorthy R

Article Info

Journal

Electrophoresis

Abbr.

Electrophoresis

ISSN

0173-0835

DOI

10.1002/elps.1150100306

Published

1989-03-00

Pages

186-9

Language

English

Region

Germany

NLM ID

8204476

Subset

External Links

PubMed source DOI: 10.1002/elps.1150100306 View journal details

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