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PMID: 2469389 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Comparison of inhibitory activities of various antiretroviral agents against particle-derived and recombinant human immunodeficiency virus type 1 reverse transcriptases.

Antimicrobial agents and chemotherapy ·Vol. 33 ·No. 1 ·1989-01-00 ·Pages 115-7

Schinazi RF, Eriksson BF, Hughes SH

Abstract

Several known antiretroviral agents were tested for their ability to inhibit the activities of human immunodeficiency virus type 1 reverse transcriptase purified from virions or from a recombinant Escherichia coli strain. The recombinant reverse transcriptase, a polypeptide of 66 kilodaltons, showed inhibition profiles indistinguishable from those of the virion-derived enzyme with all tested compounds, except for suramin and two dextran sulfates. These were more inhibitory to the recombinant enzyme, presumably because the E. coli-derived enzyme was more highly purified. The relative ease with which large quantities of recombinant enzyme can be prepared should facilitate the large-scale screening and identification of new potential inhibitors of the human immunodeficiency virus type 1 reverse transcriptase.

MeSH Terms
Antiviral Agents/pharmacology HIV-1/enzymology Recombinant Proteins Reverse Transcriptase Inhibitors
Chemicals
Antiviral Agents Recombinant Proteins Reverse Transcriptase Inhibitors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schinazi R F
Veterans Administration Medical Center, Atlanta, Georgia.
Eriksson B F
Hughes S H
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18 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.33.1.115
Published
1989-01-00
Pages
115-7
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171433
Subset
IM
Grants
NIAID NIH HHS · AI 25899 · United States
NIAID NIH HHS · AI 26055 · United States
External Links
PubMed source DOI: 10.1128/AAC.33.1.115 PMC Full Text View journal details
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