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PMID: 2469573 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Electron microscopy of U1 small nuclear ribonucleoprotein particles: shape of the particle and position of the 5' RNA terminus.

The EMBO journal ·Vol. 8 ·No. 1 ·1989-01-00 ·Pages 277-86

Kastner B, Lührmann R

Abstract

Small nuclear ribonucleoprotein particles U1 (U1 snRNPs) from HeLa cells were purified to homogeneity and examined in the electron microscope by negative staining with the double carbon film technique. A survey of several hundred views of the U1 snRNP particles revealed a main body about 8 nm in diameter with two characteristic protuberances 4-7 nm long and 3-4 nm wide. Anti-m3G antibodies were then used to define the site of the m3G base in the 5' cap of the U1 snRNA, close to the site where base-pairing occurs with the 5' splice sequence of pre-mRNA. The m3G base is located on the body of the U1 snRNP, close to one of the protuberances and approximately opposite the other.

MeSH Terms
Antibodies, Monoclonal/immunology Epitopes/analysis HeLa Cells/analysis Humans Microscopy, Electron RNA, Small Nuclear/ultrastructure Ribonucleoproteins/immunology,isolation & purification,ultrastructure Ribonucleoproteins, Small Nuclear
Chemicals
Antibodies, Monoclonal Epitopes RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kastner B
Max-Planck-Institut für Molekulare Genetik, Otto Warburg Laboratories, Berlin, FRG.
Lührmann R
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-01-00
Pages
277-86
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400800
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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