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PMID: 2470028 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Secondary structure analysis of the RepA mRNA leader transcript involved in control of replication of plasmid R1.

Nucleic acids research ·Vol. 17 ·No. 7 ·1989-04-11 ·Pages 2557-79

Ohman M, Wagner EG

Abstract

The main replication control function in plasmid R1 is an antisense RNA, CopA RNA. By binding to its target (CopT) in the leader of the RepA mRNA, CopA RNA inhibits the expression of the rate-limiting RepA protein. The formation of the RNA duplex has been proposed to alter the folding around the RepA start region. Knowledge of the secondary structure of both CopA and CopT RNA is crucial for an understanding of the regulation. Previously, we reported the structure of CopA RNA under native conditions. In the present communication we have analyzed the secondary structure of the RepA leader transcript. Our main findings are: The two loops of CopA RNA have their correspondence in CopT RNA. No major structural changes are found downstream of the duplex when CopA was bound to its target RNA during transcription. Furthermore, in agreement with CopA/CopT RNA binding studies reported recently we do not find evidence for the existence of a binding window.

MeSH Terms
Bacterial Proteins/biosynthesis,isolation & purification,metabolism Base Sequence Binding Sites DNA Helicases DNA-Binding Proteins Endoribonucleases Escherichia coli/genetics Exoribonucleases Genes, Bacterial Molecular Sequence Data Nucleic Acid Conformation Nucleotide Mapping Oligonucleotide Probes Plasmids Proteins RNA, Bacterial/biosynthesis,isolation & purification,metabolism RNA, Messenger/biosynthesis,isolation & purification,metabolism Ribonuclease H Trans-Activators Transcription, Genetic
Chemicals
Bacterial Proteins DNA-Binding Proteins Oligonucleotide Probes Proteins RNA, Bacterial RNA, Messenger Trans-Activators replication initiator protein Endoribonucleases Exoribonucleases exoribonuclease T Ribonuclease H DNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ohman M
Department of Microbiology, Uppsala University, Sweden.
Wagner E G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.7.2557
Published
1989-04-11
Pages
2557-79
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317643
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.7.2557 PMC Full Text View journal details
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