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PMID: 2471917 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Replication of cytomegalovirus in human thymic epithelial cells.

Medical microbiology and immunology ·Vol. 178 ·No. 2 ·1989-00-00 ·Pages 89-98

Numazaki K, DeStephano L, Wong I, Goldman H, Spira B, Wainberg MA

Abstract

Cytomegalovirus (CMV) has often been cited as a cause of immune suppression in children, yet little is known of the mechanisms through which this agent might affect immune function. We have succeeded in using CMV to productively infect cultured human fetal and infantile thymic epithelial (TE) cells. Morphological changes were apparent by 2-4 days after viral inoculation. CMV-related early antigen (EA) and late antigen (LA) were detected by immunofluorescence after 8 days, and progeny infectious CMV was recovered from culture media after 12-17 days. TE cells that reacted with monoclonal antibodies specific for keratin and for GQ ganglioside were predominant throughout the culture period. In contrast, infection by CMV resulted in a significant decrease in numbers of cells reactive with monoclonal antibodies specific for mesoderm-derived components. Inoculation of TE cells with CMV also caused a diminution in levels of detectable interleukin-1 (IL-1)-related antigen by 17 days after infection.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigens, Viral/analysis Child, Preschool Cytomegalovirus/growth & development,immunology Epithelium/microbiology Female Fetus Fluorescent Antibody Technique Gangliosides/analysis Humans Infant Interleukin-1/analysis Keratins/analysis Mice Pregnancy Thymus Gland/microbiology Virus Replication
Chemicals
Antibodies, Monoclonal Antigens, Viral Gangliosides Interleukin-1 Keratins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Numazaki K
Department of Microbiology, McGill University-Montreal Children's Hospital Research Institute, Montreal, Quebec, Canada.
DeStephano L
Wong I
Goldman H
Spira B
Wainberg M A
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Article Info
Journal
Medical microbiology and immunology
Abbr.
Med Microbiol Immunol
ISSN
0300-8584
DOI
10.1007/BF00203304
Published
1989-00-00
Pages
89-98
Language
English
Region
Germany
NLM ID
0314524
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00203304 View journal details
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