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PMID: 2473033 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Receptor-mediated adherence of Campylobacter pylori to mouse Y-1 adrenal cell monolayers.

Infection and immunity ·Vol. 57 ·No. 8 ·1989-08-00 ·Pages 2272-8

Evans DG, Evans DJ, Graham DY

Abstract

An in vitro adherence assay was developed to study the adherence of Campylobacter pylori to mammalian cells. Strains of C. pylori were isolated from individuals with gastritis. These strains possessed the fibrillar N-acetylneuraminyllactose(neuraminlactose)-binding hemagglutinin (NLBH) and were found to adhere to monolayers of mouse Y-1 adrenal cells. Adherence was rapid, prevented by pretreatment of the Y-1 cells with Clostridium perfringens neuraminidase, and blocked by the neuraminlactose-containing protein fetuin. Electron microscopy by the immunogold technique and with anti-NLBH antibody showed that NLBH was present at the sites of interaction between C. pylori and the Y-1 cells. These results indicate that the Y-1 monolayer is a valid model for receptor-specific adherence of C. pylori.

MeSH Terms
Adrenal Glands/microbiology,ultrastructure Animals Bacterial Adhesion/drug effects Bacterial Proteins/physiology Campylobacter/physiology,ultrastructure Cell Line Clostridium perfringens/enzymology Colony Count, Microbial Culture Techniques Kinetics Mice Neuraminidase/pharmacology Receptors, Immunologic/physiology alpha-Fetoproteins/pharmacology
Chemicals
Bacterial Proteins Receptors, Immunologic alpha-Fetoproteins bacterial adhesin receptor Neuraminidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Evans D G
Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.
Evans D J
Graham D Y
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.8.2272-2278.1989
Published
1989-08-00
Pages
2272-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313441
Subset
IM
Grants
NIDDK NIH HHS · DK-35369 · United States
NIDDK NIH HHS · DK-39919 · United States
External Links
PubMed source DOI: 10.1128/iai.57.8.2272-2278.1989 PMC Full Text View journal details
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