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PMID: 2473161 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interleukin 3-dependent and -independent mast cells stimulated with IgE and antigen express multiple cytokines.

The Journal of experimental medicine ·Vol. 170 ·No. 1 ·1989-07-01 ·Pages 245-57

Burd PR, Rogers HW, Gordon JR, Martin CA, Jayaraman S, Wilson SD, Dvorak AM, Galli SJ, Dorf ME

Abstract

In response to IgE and specific multivalent antigen, mast cell lines (both growth factor-dependent and -independent) induce the transcription and/or secretion of a number of cytokines having a wide spectrum of activities. We have identified IL-1, IL-3, IL-5, IL-6, IFN-gamma, GM-CSF, JE, MIP1 alpha, MIP1 beta, and TCA3 RNA in at least two of four mast cell clones. The production of these products (except JE) is activation-associated and can be induced by IgE plus antigen. In selected instances cytokine expression can also be induced by activation with Con A or phorbol ester plus ionophore, albeit to levels less than those observed with IgE plus antigen. In addition, long-term mast cell clones and primary cultures of bone marrow-derived mast cells specifically release IL-1, IL-4, and/or IL-6 bioactivity after activation. These findings suggest that in addition to their inflammatory effector function mast cells may serve as a source of growth and regulatory factors. The relationship of mast cells to cells of the T lymphocyte lineage is discussed.

MeSH Terms
Animals Antigens, Differentiation, B-Lymphocyte/immunology Biological Factors/biosynthesis,genetics Blotting, Northern Cell Line Cells, Cultured Clone Cells Cytokines DNA Probes Gene Expression Regulation Immunoglobulin E/immunology Interleukin-3/immunology Mast Cells/immunology,metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Nucleic Acid Hybridization RNA/genetics,isolation & purification Receptors, Fc/immunology Receptors, IgE
Chemicals
Antigens, Differentiation, B-Lymphocyte Biological Factors Cytokines DNA Probes Interleukin-3 Receptors, Fc Receptors, IgE Immunoglobulin E RNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Burd P R
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Rogers H W
Gordon J R
Martin C A
Jayaraman S
Wilson S D
Dvorak A M
Galli S J
Dorf M E
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.170.1.245
Published
1989-07-01
Pages
245-57
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189362
Subset
IM
Grants
NIAID NIH HHS · AI-22674 · United States
NCI NIH HHS · CA-09130 · United States
NCI NIH HHS · CA-39790 · United States
External Links
PubMed source DOI: 10.1084/jem.170.1.245 PMC Full Text View journal details
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