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PMID: 2473721 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Limitations of different ELISA procedures for localizing epitopes in viral coat protein subunits.

Archives of virology ·Vol. 105 ·No. 3-4 ·1989-00-00 ·Pages 269-86

Dekker EL, Porta C, Van Regenmortel MH

Abstract

The reactivity of monoclonal antibodies (McAbs) to the coat protein of tobacco mosaic virus (TMVP) with the isolated coat protein, disks, virions and a number of antigenic variants of TMV was tested in eight different ELISA procedures. Although certain McAbs, when used as detecting antibody in the liquid phase, did not react with some of these antigens, they were able to bind to them when used as the capturing antibody on the solid phase. This finding was attributed to the ability of the trapping McAb to induce a complementary conformation in the antigen presented in the liquid phase. In many cases, the reactivity of the McAbs was found to depend on the format of the ELISA. This finding together with the presence of oligomers in viral coat protein preparations made it impossible to map TMVP epitopes on the surface of the viral subunit by means of competitive ELISA.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, Viral/analysis,immunology Capsid/analysis,immunology Capsid Proteins Enzyme-Linked Immunosorbent Assay/methods Epitopes Tobacco Mosaic Virus/immunology Ultracentrifugation Viral Proteins/analysis,immunology
Chemicals
Antibodies, Monoclonal Antigens, Viral Capsid Proteins Epitopes H protein, Tobacco mosaic virus Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dekker E L
Institut de Biologie Moléculaire et Cellulaire du C.N.R.S., Strasbourg, France.
Porta C
Van Regenmortel M H
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01311363
Published
1989-00-00
Pages
269-86
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01311363 View journal details
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