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PMID: 2477245 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The PROS-35 gene encodes the 35 kd protein subunit of Drosophila melanogaster proteasome.

The EMBO journal ·Vol. 8 ·No. 8 ·1989-08-00 ·Pages 2373-9

Haass C, Pesold-Hurt B, Multhaup G, Beyreuther K, Kloetzel PM

Abstract

The proteasome is a multicatalytic proteinase complex composed of nonidentical protein subunits. We have isolated a cDNA clone encoding the 35 kd proteasome subunit of Drosophila melanogaster and propose the designation PROS-35 for the corresponding gene. The deduced amino acid sequence reveals a region of striking homology to a tyrosine phosphorylation site of viral and cellular proteins suggesting a potential regulatory function for the 35 kd subunit within the proteinase complex. Immunocytochemical experiments reveal a tissue-dependent differential distribution of the proteasome between the nucleus and cytoplasm. In addition developmental analysis shows that the proteasome is highly expressed in the CNS of stage-16 embryos and in cardia, ventriculus and ovaries of adult flies. These data suggest a tissue- and development-dependent distribution of the proteasome in D. melanogaster.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Southern Chromatography, Affinity Cysteine Endopeptidases/analysis,genetics DNA/genetics Drosophila melanogaster/enzymology,genetics Endopeptidases/analysis,genetics Immunohistochemistry Molecular Sequence Data Multienzyme Complexes/analysis,genetics Phosphorylation Poly A/genetics Proteasome Endopeptidase Complex RNA/genetics RNA, Messenger Restriction Mapping Sequence Homology, Nucleic Acid Tyrosine/metabolism
Chemicals
Multienzyme Complexes RNA, Messenger Poly A Tyrosine RNA DNA Endopeptidases Cysteine Endopeptidases Proteasome Endopeptidase Complex
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Haass C
ZMBH, Heidelberg, FRG.
Pesold-Hurt B
Multhaup G
Beyreuther K
Kloetzel P M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-08-00
Pages
2373-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401172
Subset
IM
Databases
GENBANK
X15497
External Links
PubMed source PMC Full Text View journal details
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