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PMID: 2477843 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Developmental regulation of the rat insulin-like growth factor I receptor gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 19 ·1989-10-00 ·Pages 7451-5

Werner H, Woloschak M, Adamo M, Shen-Orr Z, Roberts CT, LeRoith D

Abstract

We have investigated the developmental regulation of the rat insulin-like growth factor I (IGF-I) receptor gene in various tissues using a sensitive and specific solution hybridization/RNase protection assay. For this purpose we characterized rat IGF-I receptor cDNAs that were cloned from a simian virus 40-transformed rat granulosa cell cDNA library. The specific cDNA clone used in these studies encoded the putative signal peptide and the first 53 amino acids of the alpha subunit and was approximately 94% homologous to its human counterpart. IGF-I receptor gene expression was studied during the perinatal period and at various intervals until early adulthood. Overall, steady-state IGF-I receptor mRNA levels decreased dramatically during postnatal development; however, the extent of the decrease differed among the various tissues studied. In contrast to receptor mRNA levels, IGF-I mRNA levels increased in some of the same tissues. The molecular mechanisms underlying this apparent divergent transcriptional control of the IGF-I and IGF-I receptor genes warrant further study.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Brain/embryology Cloning, Molecular DNA/genetics Embryonic and Fetal Development Female Gene Expression Gene Expression Regulation Genes Granulosa Cells/metabolism Insulin-Like Growth Factor I/metabolism Liver/embryology Molecular Sequence Data Nucleic Acid Hybridization Poly A/genetics RNA/genetics RNA Probes RNA, Messenger/genetics Rats Rats, Inbred Strains Receptors, Cell Surface/genetics Receptors, Somatomedin Somatomedins/metabolism
Chemicals
RNA Probes RNA, Messenger Receptors, Cell Surface Receptors, Somatomedin Somatomedins Poly A RNA Insulin-Like Growth Factor I DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Werner H
Section on Molecular and Cellular Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Woloschak M
Adamo M
Shen-Orr Z
Roberts C T
LeRoith D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.19.7451
Published
1989-10-00
Pages
7451-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298082
Subset
IM
Databases
GENBANK
M27293
External Links
PubMed source DOI: 10.1073/pnas.86.19.7451 PMC Full Text View journal details
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