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PMID: 2479021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interleukin 6 induces a liver-specific nuclear protein that binds to the promoter of acute-phase genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 21 ·1989-11-00 ·Pages 8202-6

Poli V, Cortese R

Abstract

Interleukin 6 (IL-6) is responsible for a variety of biological effects related to the activation of defenses against infection or inflammation, including the immune response and the acute-phase reaction. Its mechanism of action is unknown. It has recently been shown to induce transcription of several genes encoding acute-phase proteins. Here we describe the identification of an IL-6 responsive element (IL-6RE) present in the promoter of the human hemopexin gene. This element is necessary and sufficient for the IL-6-dependent activation of transcription. The IL-6 effect does not require de novo protein synthesis. A liver-specific nuclear protein (IL-6DBP) binds to the hemopexin IL-6RE as well as to similar sequences on the promoter of other acute-phase genes. IL-6DBP DNA binding activity is induced by IL-6 via a posttranslational mechanism.

MeSH Terms
Acute-Phase Proteins/genetics Base Sequence Carcinoma, Hepatocellular Cell Line Chloramphenicol O-Acetyltransferase/genetics Genes HeLa Cells/metabolism Humans Interleukin-6/pharmacology Liver/drug effects,metabolism Liver Neoplasms Molecular Sequence Data Nuclear Proteins/biosynthesis,metabolism Oligonucleotide Probes Promoter Regions, Genetic Recombinant Proteins Transfection
Chemicals
Acute-Phase Proteins Interleukin-6 Nuclear Proteins Oligonucleotide Probes Recombinant Proteins Chloramphenicol O-Acetyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Poli V
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Cortese R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.21.8202
Published
1989-11-00
Pages
8202-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298248
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.21.8202 PMC Full Text View journal details
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