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PMID: 2481263 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An additional promoter within the protein-coding region of the psbD-psbC gene cluster in tobacco chloroplast DNA.

Nucleic acids research ·Vol. 17 ·No. 23 ·1989-12-11 ·Pages 9583-91

Yao WB, Meng BY, Tanaka M, Sugiura M

Abstract

Transcription of the psbD-psbC gene cluster in tobacco chloroplasts has been studied. This cluster contains in linear sequence the overlapping genes encoding the D2 and 43 kDa proteins of Photosystem II (psbD and psbC, respectively), and ORF62. Eight major transcripts ranging from 1.5 to 4.4 kb were detected by northern blot analysis. S1 mapping experiments revealed that these multiple transcripts comprise five distinct 5' ends whose precise positions were further determined by primer extension analysis. Two of the five 5' ends were determined to be the transcriptional initiation sites by in vitro capping assays: the main site is located 905 bp upstream from the ATG codon of psbD and the additional site is 194 bp upstream from the first ATG codon of psbC. The latter site and the preceding prokaryotic promoter motif are within the protein-coding region of psbD. The 3' ends of transcripts were determined by S1 mapping.

MeSH Terms

Base Sequence Chlorophyll/genetics DNA/genetics Genes, Plant Light-Harvesting Protein Complexes Molecular Sequence Data Multigene Family Nucleotide Mapping Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins/genetics Plants/genetics,metabolism Plants, Toxic Plasmids Promoter Regions, Genetic RNA/genetics,isolation & purification Restriction Mapping Tobacco/genetics,metabolism Transcription, Genetic

Chemicals

Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins Chlorophyll RNA DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yao W B

Center for Gene Research, Nagoya University, Japan.

Meng B Y

Tanaka M

Sugiura M

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18 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/17.23.9583

Published

1989-12-11

Pages

9583-91

Language

English

Region

England

NLM ID

0411011

PMCID

PMC335199

Subset

Databases

GENBANK

200044

External Links

PubMed source DOI: 10.1093/nar/17.23.9583 PMC Full Text View journal details

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An additional promoter within the protein-coding region of the psbD-psbC gene cluster in tobacco chloroplast DNA.

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