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PMID: 2482363 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Voltage-sensitive ion channel of Escherichia coli.

The Journal of membrane biology ·Vol. 112 ·No. 3 ·1989-12-00 ·Pages 267-75

Delcour AH, Martinac B, Adler J, Kung C

Abstract

A voltage-sensitive, cation-selective ion channel of Escherichia coli has been reconstituted into liposomes and studied with the patch-clamp method. The single channel conductance was 91 pS in symmetric solutions of 150 mM KCl. Many channels were open most of the time, with frequent brief transitions to closed levels. Multiple conducting units could close and reopen simultaneously, and this apparent cooperativity in gating was increased with depolarizing voltages. Above a voltage threshold, the channels closed irreversibly, often in groups.

MeSH Terms
Bacterial Outer Membrane Proteins/metabolism Data Interpretation, Statistical Electric Stimulation Escherichia coli/metabolism Ion Channels/metabolism Liposomes Membrane Potentials
Chemicals
Bacterial Outer Membrane Proteins Ion Channels Liposomes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Delcour A H
Department of Biochemistry, University of Wisconsin, Madison 53706.
Martinac B
Adler J
Kung C
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21 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01870957
Published
1989-12-00
Pages
267-75
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NIDDK NIH HHS · DK 39121 · United States
External Links
PubMed source DOI: 10.1007/BF01870957 View journal details
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