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PMID: 24856827 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Zinc finger and BTB domain-containing protein 3 is essential for the growth of cancer cells.

BMB reports ·Vol. 47 ·No. 7 ·2014-07-00 ·Pages 405-10

Lim JH

Abstract

ZBTB3 belongs to the Zinc finger and BTB/POZ domain containing transcription factor family; however, its biological role has rarely been studied. We demonstrate for the first time, to our knowledge, that ZBTB3 is an essential factor for cancer cell growth via the regulation of the ROS detoxification pathway. Suppression of ZBTB3 using two different short hairpin RNAs in human melanoma, lung carcinoma, and breast carcinoma results in diminished cell growth. In addition, we found that suppression of ZBTB3 activates a caspase cascade, including caspase-9, -3, and PARP leading to cellular apoptosis, resulting from failed ROS detoxification. We identified that ZBTB3 plays an important role in the gene expression of ROS detoxification enzymes. Our results reveal that ZBTB3 may play a critical role in cancer cell growth via the ROS detoxification system. Therefore, therapeutic strategies that target ZBTB3 could be used in selective cancer treatments.

MeSH Terms

Apoptosis Caspase 3/metabolism Caspase 9/metabolism Cell Line, Tumor Cell Proliferation DNA-Binding Proteins/antagonists & inhibitors,genetics,metabolism HEK293 Cells Humans Poly(ADP-ribose) Polymerases/metabolism RNA Interference RNA, Small Interfering/metabolism Reactive Oxygen Species/metabolism Repressor Proteins/antagonists & inhibitors,genetics,metabolism

Chemicals

DNA-Binding Proteins RNA, Small Interfering Reactive Oxygen Species Repressor Proteins ZBTB3 protein, human Poly(ADP-ribose) Polymerases Caspase 3 Caspase 9

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Lim Ji-Hong

Department of Biomedical Chemistry, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea.

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Article Info

Journal

BMB reports

Abbr.

BMB Rep

ISSN

1976-670X

DOI

10.5483/bmbrep.2014.47.7.075

Published

2014-07-00

Pages

405-10

Language

English

Region

Korea (South)

NLM ID

101465334

PMCID

PMC4163853

Subset

External Links

PubMed source DOI: 10.5483/bmbrep.2014.47.7.075 PMC Full Text View journal details

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Zinc finger and BTB domain-containing protein 3 is essential for the growth of cancer cells.

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