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PMID: 24884815 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Relationship amongst teratozoospermia, seminal oxidative stress and male infertility.

Reproductive biology and endocrinology : RB&E ·Vol. 12 ·2014-05-27 ·Pages 45

Agarwal A, Tvrda E, Sharma R

Abstract

Spermatozoa morphology is an important and complex characteristic of the fertilization capacity of male germ cells. Morphological abnormalities have been observed to be accompanied by reactive oxygen species (ROS) overproduction and further damage to spermatozoa, ultimately leading to infertility. Therefore, this study aimed to examine the relationship between seminal ROS production and sperm morphology in infertile teratozoospermic patients as well as in healthy men of proven and unproven fertility. Semen samples were collected from 79 patients classified as teratozoospermic and 56 healthy donors (control). Standard semen analysis was performed and spermatozoa morphology was assessed according to the WHO 2010 guidelines. Seminal ROS was measured by chemiluminescence assay. Receiver operating characteristic (ROC) curves were generated, and sensitivity, specificity, cutoff value and area under curve (AUC) were determined. Sperm morphology was significantly poor in the Teratozoospermic Group compared with the 3 Donor Groups (P < 0.05). Significantly higher levels of ROS (RLU/sec/10⁶ sperm) were seen in the Teratozoospermic group (145.4 (41.5; 555.4) compared to the Donor Groups: All Donors (64.8 (21.1; 198.2), Proven Donors (58.8 (14.2; 79.2) and Proven Donors < 2 years (58.8 (14.2; 79.2) (P < 0.05). ROS correlated negatively with sperm concentration in the All Donor group (r = -0.354; P = 0.021) as well as in the Teratozospermic group (r -0.356; P = 0.002). Using ROC analysis, we established the cutoff values for concentration, morphology and ROS. The incidence of teratozoospermia may be directly related to the overproduction of seminal ROS. Therefore, besides sperm concentration and motility, spermatozoa morphology should receive an equally important consideration in the overall assessment of male fertility.

MeSH Terms
Adult Biomarkers/metabolism Cell Shape Fertility Humans Infertility, Male/diagnosis,metabolism,pathology Male Ohio Oxidative Stress ROC Curve Reactive Oxygen Species/metabolism Semen/metabolism Semen Analysis Sensitivity and Specificity Spermatozoa/metabolism,pathology Tissue Donors Up-Regulation Young Adult
Chemicals
Biomarkers Reactive Oxygen Species
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Agarwal Ashok
Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA. [email protected].
Tvrda Eva
Sharma Rakesh
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Article Info
Journal
Reproductive biology and endocrinology : RB&E
Abbr.
Reprod Biol Endocrinol
ISSN
1477-7827
DOI
10.1186/1477-7827-12-45
Email
[email protected]
Published
2014-05-27
Epub
2014-00-27
Pages
45
Language
English
Region
England
NLM ID
101153627
PMCID
PMC4049374
Subset
IM
External Links
PubMed source DOI: 10.1186/1477-7827-12-45 PMC Full Text View journal details
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