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PMID: 2491663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Antisense RNA inhibition of beta-glucuronidase gene expression in transgenic tobacco plants.

Plant molecular biology ·Vol. 13 ·No. 4 ·1989-10-00 ·Pages 399-409

Robert LS, Donaldson PA, Ladaique C, Altosaar I, Arnison PG, Fabijanski SF

Abstract

Antisense RNA was used to specifically inhibit the expression of a GUS gene introduced in a transgenic plant. A tobacco transformant containing a single intact copy of the GUS gene and showing relatively high constitutive levels of GUS activity (GUS +) was re-transformed with an Agrobacterium Ti-derived binary vector containing an antisense version of this reporter gene. The sense and antisense GUS genes were each under the regulation of the CaMV 35S promoter. Re-transformed plants contained 1-5 copies of the antisense construct and all showed a greater than 90% reduction in GUS activity relative to the original GUS + plant. This reduction in GUS activity correlated closely with the levels of GUS enzyme and steady state GUS mRNA observed in these plants. The relatively low levels of sense and antisense GUS transcripts found in the re-transformed plants may indicate a rapid degradation of the RNA:RNA duplex in the cell.

MeSH Terms
Gene Expression Genetic Vectors Glucuronidase/genetics,metabolism Nucleic Acid Heteroduplexes/metabolism Plants/genetics,metabolism Plants, Toxic RNA, Antisense/genetics,metabolism Tobacco/genetics,metabolism Transformation, Genetic
Chemicals
Nucleic Acid Heteroduplexes RNA, Antisense Glucuronidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Robert L S
Department of Biochemistry, University of Ottawa, Ont., Canada.
Donaldson P A
Ladaique C
Altosaar I
Arnison P G
Fabijanski S F
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
Published
1989-10-00
Pages
399-409
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Corrections
ErratumIn
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