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PMID: 2492101 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

B-lymphoma cells process and present their endogenous immunoglobulin to major histocompatibility complex-restricted T cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 1 ·1989-01-00 ·Pages 282-6

Weiss S, Bogen B

Abstract

Antigen-presenting B-lymphoma cells were transfected with the gene encoding the immunoglobulin lambda 2 light chain of MOPC315 cells (lambda 2(315). The lambda 2 chain is expressed on the cell surface of the transfectants together with the endogenous heavy chain. The transfectants present an idiotope of the lambda 2(315) light chain to class II-restricted T-cell clones. Recognition by the T cells requires processing of the lambda 2(315) light chain. From these data we conclude that B-lymphoma cells constitutively process and present their immunoglobulins. Secretion and reuptake of the light chain was not necessary for the presentation. Thus, B cells bear two types of idiotypes on their membrane, a native form as surface immunoglobulin and a processed form in the context of products of the major histocompatibility complex.

MeSH Terms
Animals B-Lymphocytes/immunology Cell Line Genes, Immunoglobulin Immunoglobulin Light Chains/genetics Immunoglobulin lambda-Chains/genetics Lymphocyte Activation Lymphoma/immunology Major Histocompatibility Complex Plasmacytoma/genetics,immunology T-Lymphocytes/immunology Transfection
Chemicals
Immunoglobulin Light Chains Immunoglobulin lambda-Chains
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weiss S
Basel Institute for Immunology, Switzerland.
Bogen B
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.1.282
Published
1989-01-00
Pages
282-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286448
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.1.282 PMC Full Text View journal details
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