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PMID: 2492503 Published · ppublish English Journal Article

Duplication of the tuf gene: a new insight into the phylogeny of eubacteria.

Journal of bacteriology ·Vol. 171 ·No. 1 ·1989-01-00 ·Pages 581-4

Sela S, Yogev D, Razin S, Bercovier H

Abstract

The conservation and duplication of the tuf gene encoding the elongation factor EF-Tu were used to define phylogenetic relationships among eubacteria. When the tufA gene of Escherichia coli was used as a probe in hybridization experiments, duplicate tuf genes were found in gram-negative bacteria from three major phyla: purple bacteria, bacteroides, and cyanobacteria. Only a single copy of tuf was found in gram-positive bacteria, including mycobacteria and mycoplasmas. Gram-positive clostridia were found to carry two copies of tuf.

MeSH Terms
Bacteroides/genetics Blotting, Southern Cyanobacteria/genetics Escherichia coli/genetics Eubacterium/genetics Genes, Bacterial Nucleic Acid Hybridization Peptide Elongation Factor Tu/genetics Phylogeny
Chemicals
Peptide Elongation Factor Tu
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sela S
Department of Clinical Microbiology, Hebrew University--Hadassah Medical School, Jerusalem, Israel.
Yogev D
Razin S
Bercovier H
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.1.581-584.1989
Published
1989-01-00
Pages
581-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209627
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.1.581-584.1989 PMC Full Text View journal details
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