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PMID: 2493001 Published · ppublish English Journal Article

Thrombospondin is an osteoblast-derived component of mineralized extracellular matrix.

The Journal of cell biology ·Vol. 108 ·No. 2 ·1989-02-00 ·Pages 719-27

Robey PG, Young MF, Fisher LW, McClain TD

Abstract

Thrombospondin, the most abundant protein of platelet alpha granules, is a biosynthetic product of a variety of connective tissue cells and a component of many extracellular matrices. In this study, thrombospondin distribution in bone was investigated using a monoclonal antibody specific for the human protein. Thrombospondin was localized in osteoid of undemineralized, frozen sections of fetal subperiosteal bone, and identified as a component of mineralized bone matrix of neonatal and/or young (growing) bone of many animal species by Western blot analysis. Adult human bone cells were demonstrated to contain mRNA for thrombospondin by hybridization of a cDNA thrombospondin probe to a 6.1 kb mRNA. Pulse-chase experiments indicated that the protein was synthesized and the majority was secreted from osteoblastic cells. Treatment of the cells with TGF-beta (0.01-10 ng/ml) slightly decreased total thrombospondin synthesis, but caused an increase in the retention on newly synthesized thrombospondin in the cell layer/matrix fraction. In cell attachment assays, thrombospondin mediated adhesion, but not spreading of adult human bone cells.

MeSH Terms
Animals Blotting, Western Bone Development Bone Matrix/analysis Bone and Bones/analysis,embryology Cattle Cell Adhesion Chickens DNA Probes Dogs Glycoproteins/analysis,biosynthesis,genetics Haplorhini Humans Mice Minerals/metabolism Nucleic Acid Hybridization Osteoblasts/metabolism Quail RNA, Messenger/analysis Rabbits Rats Sheep Swine Thrombospondins Transforming Growth Factors/pharmacology
Chemicals
DNA Probes Glycoproteins Minerals RNA, Messenger Thrombospondins Transforming Growth Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Robey P G
Bone Research Branch, National Institute of Dental Research, Bethesda, Maryland 20892.
Young M F
Fisher L W
McClain T D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.2.719
Published
1989-02-00
Pages
719-27
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115449
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.108.2.719 PMC Full Text View journal details
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